| Characterization of modified and normal deoxyoligonucleotides by MALDI, time-of-flight mass spectrometry. | |
| | |
MedLine Citation:
|
PMID: 7946298 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A commercially available, matrix-assisted, laser desorption/ionization (MALDI), time-of-flight mass spectrometer has been used for the successful characterization of picomole quantities of modified deoxyoligonucleotides. The procedure was found to be applicable to the analysis of a variety of modified synthetic structures that included oligos with an intact dimethoxytrityl-blocking group, with omega-aminohexyl- and omega-hexyl-modified bases and with alterations to the normal phosphate backbone: 2'-5' and phosphorothioate linkages. Use of an alpha-cyano-4-hydroxycinnamic acid/ammonium formate based matrix gave sharp peaks, sensitivity to 1 pmol, accuracy to 0.06% on average and avoided the necessity for cryogenic technique and/or specialized equipment. Mixtures of normal and modified oligonucleotides worked well without any special precautions as long as the modified oligonucleotides contained a polyanionic backbone. Modified oligonucleotides that contained an amide linkage in place of the usual phosphate ester link required a sinapinic acid- or esculetin-based matrix with no ammonium formate. Crude oligonucleotides could be rapidly analyzed as a quality-control measure using from 10 to 20 pmol of a typical 0.2-mumol scale synthesis. |
| | |
Authors:
|
G M Hathaway |
Related Documents
:
|
12934678 - A glucosylceramide with a novel ceramide and three novel ceramides from the basidiomyce... 18618388 - Chemistry around pinene and pinane: a facile synthesis of cyclobutanes and oxatricyclo-... 18179248 - Measurement of total acid number (tan) and tan boiling point distribution in petroleum ... 18965788 - The separation of tellurium and selenium by polyurethane foam sorbents. 10699748 - Different roles for nitrogen monoxide and peroxynitrite in lipid peroxidation induced b... 16008528 - Synthesis of acylglycerols from omega-3 fatty acids and conjugated linoleic acid isomers. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: BioTechniques Volume: 17 ISSN: 0736-6205 ISO Abbreviation: BioTechniques Publication Date: 1994 Jul |
Date Detail:
|
Created Date: 1994-12-07 Completed Date: 1994-12-07 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8306785 Medline TA: Biotechniques Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 150-5 Citation Subset: IM |
Affiliation:
|
University of California, Riverside. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Base Sequence Lasers Mass Spectrometry / methods* Molecular Sequence Data Oligodeoxyribonucleotides / analysis* Quality Control |
| Chemical | |
Reg. No./Substance:
|
0/Oligodeoxyribonucleotides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A molecular technique for identification of bacteria using small subunit ribosomal RNA sequences.
Next Document: Robotic automation of dideoxyribonucleotide sequencing reactions.