Document Detail

Characterization of microrough bioactive glass surface: surface reactions and osteoblast responses in vitro.
MedLine Citation:
PMID:  12209926     Owner:  NLM     Status:  MEDLINE    
The current study characterized the in vitro surface reactions of microroughened bioactive glasses and compared osteoblast cell responses between smooth and microrough surfaces. Three different bioactive glass compositions were used and surface microroughening was obtained using a novel chemical etching method. Porous bioactive glass specimens made of sintered microspheres were immersed in simulated body fluid (SBF) or Tris solutions for 1, 6, 24, 48, or 72 h, and the formation of reaction layers was studied by means of a scanning electron microscope/energy dispersive X-ray analysis (SEM/EDXA). Cell culture studies were performed on bioactive glass disks to examine the influence of surface microroughness on the attachment and proliferation of human osteoblast-like cells (MG-63). Cell attachment was evaluated by means of microscopic counting of in situ stained cells. Cell proliferation was analyzed with a nonradioactive cell proliferation assay combined with in situ staining and laser confocal microscopy. The microroughening of the bioactive glass surface increased the rate of the silica gel layer formation during the first hours of the immersion. The formation of calcium phosphate layer was equal between control and microroughened glass surfaces. In cell cultures on bioactive glass, the microrough surface enhanced the attachment of osteoblast-like cells but did not have an effect on the proliferation rate or morphology of the cells as compared with smooth glass surface. In conclusion, microroughening significantly accelerated the early formation of surface reactions on three bioactive glasses and had a positive effect on initial cell attachment.
A Itälä; H O Ylänen; J Yrjans; T Heino; T Hentunen; M Hupa; H T Aro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research     Volume:  62     ISSN:  0021-9304     ISO Abbreviation:  J. Biomed. Mater. Res.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-09-04     Completed Date:  2003-03-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0112726     Medline TA:  J Biomed Mater Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  404-11     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Wiley Periodicals, Inc.
Department of Surgery, University of Turku, Turku, Finland.
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MeSH Terms
Biocompatible Materials*
Cell Division
Osteoblasts / cytology*
Surface Properties
Reg. No./Substance:
0/Biocompatible Materials; 7440-32-6/Titanium

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