Document Detail


Characterization of microcystins using in-source collision-induced dissociation.
MedLine Citation:
PMID:  15685685     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The efficiency of the in-source collision-induced dissociation (in-source CID) technique for the structural characterization of microcystins (MCYSTs) was evaluated. Microcystins that did not contain arginine underwent facile fragmentation to produce characteristic product ions at relatively low cone voltage and could be fully characterized based on their mass spectra. On the other hand, cyclic peptides possessing arginine residues, such as MCYST-RR, -LR, -YR and nodularin, were considerably more stable under in-source CID conditions and required higher cone voltage to induce fragmentation. This behaviour is explained in terms of the mobile proton model for peptide fragmentation that can be used as an indication for the presence of arginine when unknown microcystins are analyzed. In-source CID was applied to the characterization of microcystins released into water from a Microcystis aeruginosa culture (UTCC299) (UTCC: University of Toronto Culture Collection of Algae and Cyanobacteria). Six microcystins were detected in extracts from UTCC299: I, [D-Asp(3)]MCYST-LR; II, MCYST-LR; III, isomer of MCYST-LR; IV, isomer of methyl MCYST-LR; V, [D-Asp(3), Glu(OCH(3))(6)]MCYST-LR; and VI, [D-Glu(OCH(3))(6)]MCYST-LR. In-source CID provided mass spectral patterns similar to those obtained by CID in the collision cell of the mass spectrometer but was more sensitive for the analysis of microcystins.
Authors:
Cariton Kubwabo; Natalia Vais; Frank M Benoit
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  19     ISSN:  0951-4198     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2005  
Date Detail:
Created Date:  2005-02-17     Completed Date:  2005-05-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  597-604     Citation Subset:  IM    
Affiliation:
Chemistry Research Division, Safe Environments Programme, Health Canada, Environmental Health Centre, Ottawa, Ontario K1A 0L2, Canada. Cariton_Kubwabo@hc-sc.gc.ca
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Liquid / methods*
Microcystins
Microcystis / metabolism*
Peptides, Cyclic / analysis*,  chemistry*
Spectrometry, Mass, Electrospray Ionization / methods*
Chemical
Reg. No./Substance:
0/Microcystins; 0/Peptides, Cyclic; 77238-39-2/microcystin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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