| Characterization of methanotrophic bacteria on the basis of intact phospholipid profiles. | |
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MedLine Citation:
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PMID: 10913867 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The intact phospholipid profiles (IPPs) of seven species of methanotrophs from all three physiological groups, type I, II and X, were determined using liquid chromatography/electrospray ionization/mass spectrometry. In these methanotrophs, two major classes of phospholipids were found, phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) as well as its derivatives phosphatidylmethylethanolamine (PME) and phosphatidyldimethylethanolamine (PDME). Specifically, the type I methanotrophs, Methylomonas methanica, Methylomonas rubra and Methylomicrobium album BG8 were characterized by PE and PG phospholipids with predominantly C16:1 fatty acids. The type II methanotrophs, Methylosinus trichosporium OB3b and CSC1 were characterized by phospholipids of PG, PME and PDME with predominantly C18:1 fatty acids. Methylococcus capsulatus Bath, a representative of type X methanotrophs, contained mostly PE (89% of the total phospholipids). Finally, the IPPs of a recently isolated acidophilic methanotroph, Methylocella palustris, showed it had a preponderance of PME phospholipids with 18:1 fatty acids (94% of total). Principal component analysis showed these methanotrophs could be clearly distinguished based on phospholipid profiles. Results from this study suggest that IPP can be very useful in bacterial chemotaxonomy. |
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Authors:
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J Fang; M J Barcelona; J D Semrau |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: FEMS microbiology letters Volume: 189 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2000 Aug |
Date Detail:
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Created Date: 2000-08-29 Completed Date: 2000-08-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 67-72 Citation Subset: IM |
Affiliation:
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Department of Civil and Environmental Engineering, The University of Michigan, Ann Arbor 48109, USA. jsfang@engin.umich.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Typing Techniques Chromatography, Liquid Mass Spectrometry / methods Methylococcaceae / chemistry*, classification* Phospholipids / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Phospholipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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