Document Detail

Characterization of the metabolite produced by Mycosphaerella pinodes, the causal agent of mycosphaerella blight on field peas (Pisum sativum L.).
MedLine Citation:
PMID:  15462518     Owner:  NLM     Status:  MEDLINE    
The metabolite produced by Mycosphaerella pinodes, the causal agent of mycosphaerella blight on field peas, was detected by thin layer chromatography (TLC) and was analyzed for its chemical and pathogenic characteristics. One blue dot was detected using 254nm UV light on TLC plate, and a spray of rho-anisaldehyde (110 degrees C, 30 min) also produced a blue dot. The solvent systems used for TLC analysis were ethyl acetate/water/acetone (5/2/5), chloroform/methanol/glacial acetic acid (19/10/2), toluene/ethyl acetate/90% formic acid (6/3/1), diethylether/methanol/water/90% formic acid (95/4/1/1), and bezene/methanol/acetic acid (24/2/1), with R(f) values (min-max) of 0.09-0.18, 0.88-0.95, 0.06-0.15, 0.39-0.47 and 0.05-0.12, respectively. The recovered metabolite from the TLC plate displayed UV absorption peaks at 212, 244, 250, 256 and 261 nm. The proposed formula of the main component of the metabolite was C16H12N3O6. The TLC-purified metabolite induced symptom of discoloration on detached pea leaves.
S F Hwang; H Su; K F Chang; G D Turnbull; S F Blade; R J Howard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbiological research     Volume:  159     ISSN:  0944-5013     ISO Abbreviation:  Microbiol. Res.     Publication Date:  2004  
Date Detail:
Created Date:  2004-10-06     Completed Date:  2004-11-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9437794     Medline TA:  Microbiol Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  187-91     Citation Subset:  IM    
Alberta Research Council, Post Bag 4000, Vegreville, AB T9C 1T4, Canada.
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MeSH Terms
Ascomycota / metabolism*,  pathogenicity*
Chromatography, Thin Layer
Mycotoxins / chemistry,  metabolism,  toxicity*
Peas / microbiology*
Plant Diseases / microbiology*
Plant Leaves / microbiology
Reg. No./Substance:

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