Document Detail


Characterization of melanoma cell membrane tumor-associated antigens using xenoserum, alloserum, and autoserum: I. immunofluorescence.
MedLine Citation:
PMID:  7003264     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Xenoantiserum, alloantiserum, and autoantiserum prepared against human melanoma cells from cell lines maintained in tissue culture were evaluated by indirect immunofluorescence (ind IF) for their content of antibodies reactive with melanoma cell membrane tumor-associated antigens (TAA). Following absorptions which removed all detectable anti-human species, anti-RBC, and anti-HLA antigen activity, the antisera were tested against a panel of human cell lines. Three apparently distinct melanoma-associated cell membrane antigens were detected. The first of these was present on all human cells maintained in tissue culture including cells of both malignant and nonmalignant origin. The second antigen was expressed on all cells of malignant origin and also by fetal fibroblasts. The third antigen was detectable only on melanoma cells and was expressed by a majority of the melanomas tested. No evidence was obtained to suggest the presence of melanoma TAA unique to a single melanoma cell line. Marked differences were observed in the antigenic profile detected by the three antisera.
Authors:
P A Mutzner; G M Stuhlmiller; H F Seigler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of surgical oncology     Volume:  14     ISSN:  0022-4790     ISO Abbreviation:  J Surg Oncol     Publication Date:  1980  
Date Detail:
Created Date:  1981-02-24     Completed Date:  1981-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0222643     Medline TA:  J Surg Oncol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  367-77     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Neoplasm / analysis*
Cell Membrane / analysis
Cells, Cultured
Cytotoxicity Tests, Immunologic
Fetus
Fibroblasts / analysis
Fluorescent Antibody Technique
Humans
Immune Sera / analysis
Melanoma / immunology*
Pan troglodytes
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Immune Sera

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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