Document Detail

Characterization of melanoma cell membrane tumor-associated antigens using xenoserum, alloserum, and autoserum: I. immunofluorescence.
MedLine Citation:
PMID:  7003264     Owner:  NLM     Status:  MEDLINE    
Xenoantiserum, alloantiserum, and autoantiserum prepared against human melanoma cells from cell lines maintained in tissue culture were evaluated by indirect immunofluorescence (ind IF) for their content of antibodies reactive with melanoma cell membrane tumor-associated antigens (TAA). Following absorptions which removed all detectable anti-human species, anti-RBC, and anti-HLA antigen activity, the antisera were tested against a panel of human cell lines. Three apparently distinct melanoma-associated cell membrane antigens were detected. The first of these was present on all human cells maintained in tissue culture including cells of both malignant and nonmalignant origin. The second antigen was expressed on all cells of malignant origin and also by fetal fibroblasts. The third antigen was detectable only on melanoma cells and was expressed by a majority of the melanomas tested. No evidence was obtained to suggest the presence of melanoma TAA unique to a single melanoma cell line. Marked differences were observed in the antigenic profile detected by the three antisera.
P A Mutzner; G M Stuhlmiller; H F Seigler
Related Documents :
630534 - Primary leptomeningeal melanoma: an ultrastructural study.
15158544 - Anatomy of the sweat glands, pharmacology of botulinum toxin, and distinctive syndromes...
6385004 - Localization of the gangliosides gd2 and gd3 in adhesion plaques and on the surface of ...
10763124 - Flow cytometric estimation of the plasma membrane diversity of transplantable melanomas...
24188694 - Wnt signaling blockage inhibits cell proliferation and migration, and induces apoptosis...
17460564 - Effects of activin a on pancreatic ductal cells in streptozotocin-induced diabetic rats.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of surgical oncology     Volume:  14     ISSN:  0022-4790     ISO Abbreviation:  J Surg Oncol     Publication Date:  1980  
Date Detail:
Created Date:  1981-02-24     Completed Date:  1981-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0222643     Medline TA:  J Surg Oncol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  367-77     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antigens, Neoplasm / analysis*
Cell Membrane / analysis
Cells, Cultured
Cytotoxicity Tests, Immunologic
Fibroblasts / analysis
Fluorescent Antibody Technique
Immune Sera / analysis
Melanoma / immunology*
Pan troglodytes
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Immune Sera

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The role of the Escherichia coli lambda receptor in the transport of maltose and maltodextrins.
Next Document:  Hyporeninemic hypoaldosteronism. Case report and attempt at pathophysiological classification (autho...