Document Detail

Characterization of melanin-concentrating hormone from rat hypothalamus.
MedLine Citation:
PMID:  2759038     Owner:  NLM     Status:  MEDLINE    
A melanin-concentrating hormone (MCH)-like peptide was isolated from rat hypothalamus by acid extraction, gel filtration chromatography, immunoaffinity chromatography using antiserum directed against salmon MCH, and two steps of HPLC using octadecyl columns. Several zones of immunoreactivity were isolated, and Edman degradation in a gas phase sequencer indicated that the amino acid sequence of all zones was identical. Rat hypothalamic MCH is a nonadecapeptide which differs from salmon MCH by an N-terminal extension of two amino acids and four additional substitutions. Rat MCH has the following primary structure: Asp-Phe-Asp-Met-Leu-Arg-Cys-Met-Leu-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Gln- Val.
J M Vaughan; W H Fischer; C Hoeger; J Rivier; W Vale
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  125     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1989 Sep 
Date Detail:
Created Date:  1989-09-20     Completed Date:  1989-09-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1660-5     Citation Subset:  AIM; IM    
Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, San Diego, California 92138-9216.
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MeSH Terms
Amino Acid Sequence
Amino Acids / analysis
Chromatography, Affinity
Chromatography, Gel
Chromatography, High Pressure Liquid
Hypothalamic Hormones*
Hypothalamus / analysis*
Melanins / isolation & purification*
Molecular Sequence Data
Pituitary Hormones / isolation & purification*
Sequence Homology, Nucleic Acid
Grant Support
Reg. No./Substance:
0/Amino Acids; 0/Hypothalamic Hormones; 0/Melanins; 0/Pituitary Hormones; 67382-96-1/melanin-concentrating hormone

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