Document Detail

Characterization of medium chain length (R)-3-hydroxycarboxylic acids produced by Streptomyces sp. JM3 and the evaluation of their antimicrobial properties.
MedLine Citation:
PMID:  22806719     Owner:  NLM     Status:  Publisher    
(R)-3-Hydroxycarboxylic acids, chiral enantiomers of bacterial polyhydroxyalkanoates (PHA), may be valuable synthons for the production of numerous industrial materials such as β-lactams, fungicides, flavors, pheromones and vitamins. In this study, (R)-3-hydroxycarboxylic acid [(R)-3HAs)] synthons were produced by Streptomyces sp. JM3 (JN166713) under batch fermentation. Initial confirmation of PHA production was achieved by matrix assisted laser desorption ionization-time of flight mass spectroscopy and gas chromatography/mass spectroscopy (GC/MS). Subsequently, (R)-3HAs were produced by in vivo depolymerization and the monomers were separated using acid precipitation and anion exchange chromatography. The (R)-3HAs were identified by GC/MS as 3-trimethylsiloxy esters of decanoic, octanoic and butanoic acids. This was further supported by (13)C nuclear magnetic resonance spectrometry. The (R)-3HAs exhibited antimicrobial activity against Escherichia coli O157:H7, Listeria monocytogenes (ATCC 7644) and Salmonella typhimurium (ATCC 14028) with minimum inhibitory concentration ranging from 12.5 to 25 mg ml(-1). However, the minimum bactericidal concentration data suggest that the (R)-3HAs may be bactericidal for E. coli O157:H7 and bacteriostatic for S. typhimurium and L. monocytogenes. Furthermore, the major purified synthon was shown to minimize the invasion of fibroblasts by S. typhimurium (ATCC 14028) [p < 0.05], using the MTT assay [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)].
Adrian Douglas Allen; Patrick Daley; Folahan O Ayorinde; Ayelle Gugssa; Winston A Anderson; Broderick E Eribo
Related Documents :
16509599 - Novel 99mtc-labeled neurotensin analogues with optimized biodistribution properties.
10624869 - Phytochemical study of cuachalalate (amphiptherygium adstringens, schiede ex schlecht).
10727939 - Formation of a new class of oxylipins from n-acyl(ethanol)amines by the lipoxygenase pa...
2229019 - Three-dimensional structure of an alpha-amylase inhibitor haim as determined by nuclear...
25236779 - Beef quality traits of heifer in comparison with steer, bull and cow at various feeding...
315709 - Structural difference between the normal pim1 and the common pim2 variant of human alph...
10425139 - Glaziovianol, a new terpenoid hydroquinone from auxemma glazioviana
25039349 - Perilipin 3 modulates specific lipogenic pathways in sz95 sebocytes.
886389 - Lack of effect of ascorbic and citric acids on calcium metabolism of chickens.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-19
Journal Detail:
Title:  World journal of microbiology & biotechnology     Volume:  -     ISSN:  1573-0972     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-7-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9012472     Medline TA:  World J Microbiol Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Biology, Howard University, 415 College St. NW, Washington, DC, 20059, USA,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Construction of a stable genetically engineered rhamnolipid-producing microorganism for remediation ...
Next Document:  Characteristics of aerobic and anaerobic ammonium-oxidizing bacteria in the hyporheic zone of a cont...