Document Detail


Characterization and mapping of sleep-waking specific neurons in the basal forebrain and preoptic hypothalamus in mice.
MedLine Citation:
PMID:  19285545     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We recorded 872 single units across the complete sleep-waking cycle in the mouse preoptic area (POA) and basal forebrain (BFB), which are deeply involved in the regulation of sleep and wakefulness (W). Of these, 552 were sleep-active, 96 were waking-active, 106 were active during both waking and paradoxical sleep (PS), and the remaining 118 were state-indifferent. Among the 872, we distinguished slow-wave sleep (SWS)-specific, SWS/PS-specific, PS-specific, W-specific, and W/PS-specific neurons, the last group being further divided into specific tonic type I slow (TI-Ss) and specific tonic type I rapid (TI-Rs) both discharging specifically in association with cortical activation during both W and PS. Both the SWS/PS-specific and PS-specific neurons were distributed throughout a wide region of the POA and BFB, whereas the SWS-specific neurons were mainly located in the middle and ventral half of the POA and adjacent BFB, as were the W-specific and W/PS-specific neurons. At the transition from waking to sleep, the majority of SWS-specific and all SWS/PS-specific neurons fired after the onset of cortical synchronization (deactivation), whereas all W-specific and W/PS-specific neurons showed a significant decrease in firing rate >0.5 s before the onset. At the transition from SWS to W, the sleep-specific neurons showed a significant decrease in firing rate 0.1 s before the onset of cortical activation, while the W-specific and W/PS-specific neurons fired >0.5 s before the onset. TI-Ss neurons were characterized by a triphasic broad action potential, slow single isolated firing, and an antidromic response to cortical stimulation, whereas TI-Rs neurons were characterized by a narrow action potential and high frequency burst discharge in association with theta waves in PS. These data suggest that the forebrain sleep/waking switch is regulated by opposing activities of sleep-promoting (SWS-specific and SWS/PS-specific) and waking-promoting (W-specific and W/PS-specific) neurons, that the initiation of sleep is caused by decreased activity of the waking-promoting neurons (disfacilitation), and that the W/PS-specific neurons are deeply involved in the processes of cortical activation/deactivation.
Authors:
K Takahashi; J-S Lin; K Sakai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-12
Journal Detail:
Title:  Neuroscience     Volume:  161     ISSN:  1873-7544     ISO Abbreviation:  Neuroscience     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-11     Completed Date:  2009-09-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  269-92     Citation Subset:  IM    
Affiliation:
INSERM U628, Lyon, F-69373, France and Claude Bernard University Lyon 1, 8 Avenue Rockefeller, Lyon Cedex 08, F-69373, France.
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MeSH Terms
Descriptor/Qualifier:
Acoustic Stimulation
Action Potentials
Animals
Brain Mapping
Electric Stimulation
Male
Mice
Mice, Inbred C57BL
Neurons / physiology*
Preoptic Area / cytology,  physiology
Prosencephalon / cytology*,  physiology
Sleep*
Sleep Stages
Wakefulness*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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