| Characterization of the major parathyroid hormone target cell in the endosteal metaphysis of rat long bones. | |
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MedLine Citation:
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PMID: 2080716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The majority of in vivo competitive binding of parathyroid hormone (PTH) in the endosteal metaphysis of rat long bones was recently shown to be localized in the intertrabecular tissue to a cell that is distinct from a differentiated osteoblast. In the present report we have further characterized this cell, termed a parathyroid hormone target (PT) cell, by light and electron microscopy using radioautography and histochemical techniques. These studies demonstrate that the PT cell is a mononuclear cell with a large cell body located at times between clusters of differentiated osteoblasts, as well as in other regions of the intertrabecular tissue. Its long cytoplasmic processes extend from the bone matrix through the intertrabecular region toward vascular structures, interdigitating with various cells of the endosteum. A distinctive tubular structure originating in the Golgi system and often associated with long mitochondria and glycogen particles extends throughout the cytoplasmic processes of the PT cell. Based on its capacity to incorporate [3H]thymidine, the PT cell appears to divide rather slowly. The identification of occasional hybrid cells with ultrastructural features of both the PT cell and the differentiated osteoblast and the presence of histochemical evidence for alkaline phosphatase activity suggest that the PT cell is of the osteoblast lineage. These studies therefore morphologically define a major osseous target cell for PTH that, although of the osteoblast lineage, is not a differentiated osteoblast and provide in vivo evidence that characteristics of the "osteoblast phenotype" are not restricted to a sole osseous cell type. |
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Authors:
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M F Rouleau; J Mitchell; D Goltzman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research Volume: 5 ISSN: 0884-0431 ISO Abbreviation: J. Bone Miner. Res. Publication Date: 1990 Oct |
Date Detail:
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Created Date: 1991-04-26 Completed Date: 1991-04-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8610640 Medline TA: J Bone Miner Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1043-53 Citation Subset: IM |
Affiliation:
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Calcium Research Laboratory, McGill University, Montreal, Quebec, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoradiography Binding, Competitive / physiology Bone and Bones / cytology, metabolism*, ultrastructure Cell Division / physiology Male Monocytes / metabolism, ultrastructure Parathyroid Hormone / metabolism* Protein Binding Rats Rats, Inbred Strains Thymidine / metabolism Tritium / diagnostic use |
| Chemical | |
Reg. No./Substance:
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0/Parathyroid Hormone; 10028-17-8/Tritium; 50-89-5/Thymidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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