| Characterization of major elements of insulin signaling cascade in chicken adipose tissue: Apparent insulin refractoriness. | |
| | |
MedLine Citation:
|
PMID: 22233773 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The role of insulin in chicken adipose tissue appears weak or questionable. In a first study, proximal and distal components of the insulin signaling cascade were characterized in abdominal adipose tissue of fasted or fed chickens for the first time. Similar measurements were performed on epididymal adipose tissue from fasted or fed rats for comparison. Tyrosine phosphorylation of IR beta subunit, IRS-1 and Shc and phosphorylation of downstream components (Akt and MAPK ERK1/2) were significantly reduced as expected by fasting in rat, but not in chicken. Phosphorylation of MAPK P38 was increased by fasting in chicken but not in rat. Phosphorylation of AMPK was not affected in the conditions investigated in either species. Whatever the nutritional state, the protein levels of IR and IRS-1 were lower in chicken than in rat, whereas those of Shc, Akt, AMPK, MAPK ERK2 and MAPK P38 were similar in both species. In fed state, PI3K activity was higher in chicken than in rat. Insulin sensitivity of insulin cascade was further investigated in chicken adipose tissue following in vivo insulin neutralization for 1 or 5h in fed chickens. Insulin privation did not alter early insulin signaling steps (IRβ, IRS-1 and Shc) or downstream elements (Akt, P70S6K, S6 ribosomal protein, AMPK, MAPK ERK2 and MAPK P38). Finally, phosphorylation of the transcription factor Creb was increased by 2-fold by 5h fasting or 5h insulin privation, most likely in response to an increase in plasma glucagon levels. Thus, insulin signaling is markedly different in chicken abdominal adipose tissue from that operating in mammals making chicken an interesting model of insulin resistance or refractoriness. |
| | |
Authors:
|
Joëlle Dupont; Sonia Métayer-Coustard; Bo Ji; Christelle Ramé; Christian Gespach; Brynn Voy; Jean Simon |
Related Documents
:
|
21134603 - Chromium (d-phenylalanine)3 alleviates high fat-induced insulin resistance and lipid ab... 21134293 - Low-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio is th... 15318993 - The enteroinsular axis and the recovery from type 2 diabetes after bariatric surgery. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-3 |
Journal Detail:
|
Title: General and comparative endocrinology Volume: - ISSN: 1095-6840 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-11 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370735 Medline TA: Gen Comp Endocrinol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012. Published by Elsevier Inc. |
Affiliation:
|
Unité de Physiologie de la Reproduction et des Comportements (UMR85), INRA, 37380 Nouzilly, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Central and peripheral glucocorticoid receptors are involved in the plasma cortisol response to an a...
Next Document: Body weight and leukocyte infiltration after an acute exercise-related muscle injury in ovariectomiz...