| Characterization of lymphocyte fibronectin. | |
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MedLine Citation:
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PMID: 8598219 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In vitro cultured "activated" peripheral blood lymphocytes and T-cell lines synthesized a high-molecular-weight gelatin binding molecule (MW 500 kDa), whereas resting lymphocytes showed poor or negligible synthesis of the same component. Concanavalin A-mediated anchorage of the lymphocytes to a substratum potentiated synthesis of the high-molecular-weight molecule. Western blotting of the gelatin-binding lymphocyte molecule demonstrated reactivity with antibodies specific for human fibronectin. Furthermore, immunocytochemistry showed reactivity of anti-fibronectin antibodies with T-lymphocytes at the single-cell level. The lymphocyte-derived fibronectin was preferentially cell associated and relatively small amounts were present in the culture medium. RT-PCR of total RNA from CD4+ T-cells and the lymphoid T-cell line MOLT-4 showed that the most abundant species of fibronectin mRNA lacked the entire III CS exon encoding the alpha 4 beta 1 binding region LDV. Amplification of the III CS region from other T-cell lines revealed that these cells expressed several fibronectin mRNA isoforms most of which were lacking the LDV coding sequence. In conclusion, synthesis of fibronectin is demonstrated to occur in T-lymphocytes and to be regulated by signals which activate the cells. |
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Authors:
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D Hauzenberger; N Martin; S Johansson; K G Sundqvist |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental cell research Volume: 222 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1996 Feb |
Date Detail:
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Created Date: 1996-04-19 Completed Date: 1996-04-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 312-8 Citation Subset: IM |
Affiliation:
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Department of Clinical Immunology, Karolinska Institute, Huddinge Hospital, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Alternative Splicing / immunology Amino Acid Sequence Cell Adhesion / genetics, immunology Fibronectins / analysis*, genetics, immunology Humans Immunohistochemistry Lymphocyte Activation / immunology Molecular Sequence Data Oligopeptides RNA Precursors / analysis T-Lymphocytes / chemistry*, physiology |
| Chemical | |
Reg. No./Substance:
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0/Fibronectins; 0/Oligopeptides; 0/RNA Precursors; 125850-12-6/leucyl-aspartyl-valine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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