Document Detail


Characterization of low-density lipoprotein uptake by murine macrophages exposed to Chlamydia pneumoniae.
MedLine Citation:
PMID:  10602673     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Exposure to Chlamydia pneumoniae is correlated with atherosclerosis in a variety of clinical and epidemiological studies, but how the organism may initiate and promote the disease is poorly understood. One pathogenic mechanism could involve modulation of macrophage function by C. pneumoniae. We recently demonstrated that C. pneumoniae induces macrophages to accumulate excess cholesterol and develop into foam cells, the hallmark of early atherosclerotic lesions. To determine if C. pneumoniae-induced foam cell formation involved increased uptake of low-density lipoprotein (LDL), the current study examined macrophage association of a fluorescent carbocyanine (DiI)-labeled LDL following infection. C. pneumoniae enhanced the association of DiI-LDL with macrophages in a dose-dependent manner with respect to both C. pneumoniae and DiI-LDL. Interestingly, increased association was inhibited by native LDL and occurred in the absence of oxidation byproducts and in the presence of antioxidants. However, enhanced DiI-LDL association occurred without the participation of the classical Apo B/E native LDL receptor, since C. pneumoniae increased DiI-LDL association and induced foam cell formation in macrophages isolated from LDL-receptor-deficient mice. Surprisingly, DiI-LDL association was inhibited not only by unlabeled native LDL but also by high-density lipoprotein, very low density lipoprotein, and oxidized LDL. These data indicate that exposure of macrophages to C. pneumoniae increases the uptake of LDL and foam cell formation by an LDL-receptor-independent mechanism.
Authors:
M V Kalayoglu; G S Miranpuri; D T Golenbock; G I Byrne
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Microbes and infection / Institut Pasteur     Volume:  1     ISSN:  1286-4579     ISO Abbreviation:  Microbes Infect.     Publication Date:  1999 May 
Date Detail:
Created Date:  2000-06-16     Completed Date:  2000-06-16     Revised Date:  2014-03-20    
Medline Journal Info:
Nlm Unique ID:  100883508     Medline TA:  Microbes Infect     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  409-18     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbocyanines / metabolism
Cell Line
Cells, Cultured
Chlamydophila pneumoniae / pathogenicity*
Fluorescence
Foam Cells / cytology*,  metabolism,  microbiology
Lipoproteins, LDL / metabolism*
Macrophages / metabolism,  microbiology*
Macrophages, Peritoneal / metabolism,  microbiology
Mice
RNA, Messenger / metabolism
Receptors, LDL / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
AI42790/AI/NIAID NIH HHS; R01 AI042790/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Carbocyanines; 0/Lipoproteins, LDL; 0/RNA, Messenger; 0/Receptors, LDL
Comments/Corrections
Comment In:
CNS Neurosci Ther. 2010 Spring;16(1):1-2   [PMID:  20070784 ]

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