Document Detail


Characterization and location of secretory phospholipase A2 groups IIE, V, and X in the rat brain.
MedLine Citation:
PMID:  16511882     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Secretory phospholipases A(2) (sPLA(2)) form a diverse family of enzymes involved in a variety of physiologic and pathologic processes. Common among all sPLA(2) is the ability to cleave the second position of phospholipids, thereby releasing fatty acid and a lysophospholipid. Several sPLA(2) have been cloned and characterized in various tissues and receptors have been identified. In the nervous system, sPLA(2) groups IB, IIA, IIE, IIF, V, and XII have been identified, and binding sites for sPLA(2) have been found. Here, we report sPLA(2)-IIE and sPLA(2)-X in rat brain as well as in neurons in primary culture. We furthermore confirm the presence of sPLA(2)-V in rat brain and demonstrate the presence of sPLA(2)-V in primary neuronal cultures. The distribution of sPLA(2)-IIE, V, and -X seems to be mainly neuronal, with the highest abundance occurring in the cerebral cortex and hippocampus. We also find that sPLA(2)-IIE, -V, and -X are differentially induced by kainic acid. This study supports the concept that sPLA(2) heterogeneity in brain is functionally relevant and responsive to seizures.
Authors:
Miriam Kolko; Nanna R Christoffersen; Sebastian G Barreiro; Martin L Miller; Andrew J Pizza; Nicolas G Bazan
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  83     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-23     Completed Date:  2006-06-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  874-82     Citation Subset:  IM    
Affiliation:
Neuroscience Center of Excellence, Department of Ophthalmology, Louisiana State University Health Sciences Center School of Medicine, New Orleans, Louisiana 70112, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / physiology*
Cells, Cultured
Embryo, Mammalian
Group II Phospholipases A2
Immunohistochemistry
In Situ Hybridization
Neurons / physiology*
Phospholipases A / genetics*,  metabolism*
Phospholipases A2
Rats
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
NS23002/NS/NINDS NIH HHS; NS46741/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Group II Phospholipases A2; EC 3.1.1.4/Phospholipases A2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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