Document Detail

Characterization and localization of side population (SP) cells in zebrafish kidney hematopoietic tissue.
MedLine Citation:
PMID:  17932252     Owner:  NLM     Status:  MEDLINE    
We previously showed that side population (SP) cells, characterized by specific Hoechst dye efflux pattern in flow cytometric analysis, were present in teleost kidney hematopoietic tissue, and that kidney SP cells were enriched in hematopoietic stem cells (HSCs). ABCG2/Abcg2 is an ATP-binding cassette (ABC) transporter that is known to be associated with Hoechst dye efflux activity of mammalian HSCs. In the present study, we examined the expression and function of Abcg2 in kidney SP cells from zebrafish (Danio rerio). Although the zebrafish genome has 4 paralogous copies of ABCG2 (zAbcg2a, b, c, and d), zAbcg2a and zAbcg2c mRNA was expressed in kidney SP cells. Transfection of COS-7 cells with zAbcg2a and zAbcg2c showed that zAbcg2a was directly associated with the SP phenotype. These results indicate that zAbcg2a mRNA is a useful marker for zebrafish HSCs. In situ hybridization in kidney tissue showed that zAbcg2a-positive cells were sporadically localized on the surface of renal tubules, and tightly adhered to renal tubule epithelial cells. This result suggests that teleost HSCs adhere to the surface of renal tubules, and that renal tubule epithelial cells are a key component of HSC niche in teleosts.
Isao Kobayashi; Kazuyuki Saito; Tadaaki Moritomo; Kyosuke Araki; Fumio Takizawa; Teruyuki Nakanishi
Publication Detail:
Type:  Journal Article     Date:  2007-10-11
Journal Detail:
Title:  Blood     Volume:  111     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-28     Completed Date:  2008-03-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1131-7     Citation Subset:  AIM; IM    
Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa, Japan.
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MeSH Terms
ATP-Binding Cassette Transporters / chemistry,  classification,  genetics,  metabolism
Amino Acid Sequence
COS Cells
Cercopithecus aethiops
Conserved Sequence
Gene Expression Regulation
Hematopoietic Stem Cells / cytology*,  metabolism*
Kidney / cytology*,  metabolism*
Molecular Sequence Data
Sequence Alignment
Zebrafish / genetics,  metabolism*
Reg. No./Substance:
0/ATP-Binding Cassette Transporters

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