Document Detail

Characterization of a liver metastatic variant of murine colon 26 carcinoma cells.
MedLine Citation:
PMID:  9222309     Owner:  NLM     Status:  MEDLINE    
Intraportal vein injection of highly metastatic L5 cells consistently resulted in liver metastases (increases in the number of tumor colonies in the liver), whereas inoculation of P cells rarely did. L5 cells invaded the basement membrane Matrigel in greater numbers than did P cells, suggesting that the metastatic potential of L5 cells is partly related to enhanced invasive properties. The enhanced adhesion of L5 cells to fibronectin-, laminin- and Matrigel-coated substrates, as well as their haptotactic migration to fribronectin, may be associated with the preferential expression of VLA-2 and VLA-4 integrins on the surface of these cells detected by flow cytometry. Gelatin zymograms showed that the degradative activity of 72-kD gelatinases was greater in L5 cells than P cells. These results indicate that, in addition to adhesiveness and motility, the invasive ability of L5 cells may also be attributed to enhanced gelatinolytic activity. L5 cells grew more rapidly than P cells in vitro. Thus, an experimental model using highly metastatic colon 26 L5 cells would be useful for analyzing the molecular mechanism of liver metastasis and for evaluating the efficacy of treatment of occult micrometastases which may already have been disseminated at the time of surgery.
Y Ohnishi; T Sakamoto; H Fujii; F Kimura; J Murata; K Tazawa; M Fujimaki; Y Sato; M Kondo; Y Une; J Uchino; I Saiki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine     Volume:  18     ISSN:  1010-4283     ISO Abbreviation:  Tumour Biol.     Publication Date:  1997  
Date Detail:
Created Date:  1997-08-05     Completed Date:  1997-08-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8409922     Medline TA:  Tumour Biol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  113-22     Citation Subset:  IM    
Department of Pathogenic Biochemistry, Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University, Japan.
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MeSH Terms
Adenocarcinoma / pathology,  secondary*
Cell Adhesion
Cell Movement
Colonic Neoplasms / pathology*
Integrin alpha4beta1
Integrins / physiology
Liver Neoplasms / secondary*
Mice, Inbred BALB C
Neoplasm Invasiveness
Receptors, Collagen
Receptors, Lymphocyte Homing / physiology
Specific Pathogen-Free Organisms
Reg. No./Substance:
0/Integrin alpha4beta1; 0/Integrins; 0/Receptors, Collagen; 0/Receptors, Lymphocyte Homing

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