| Characterization of a liver metastatic variant of murine colon 26 carcinoma cells. | |
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MedLine Citation:
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PMID: 9222309 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intraportal vein injection of highly metastatic L5 cells consistently resulted in liver metastases (increases in the number of tumor colonies in the liver), whereas inoculation of P cells rarely did. L5 cells invaded the basement membrane Matrigel in greater numbers than did P cells, suggesting that the metastatic potential of L5 cells is partly related to enhanced invasive properties. The enhanced adhesion of L5 cells to fibronectin-, laminin- and Matrigel-coated substrates, as well as their haptotactic migration to fribronectin, may be associated with the preferential expression of VLA-2 and VLA-4 integrins on the surface of these cells detected by flow cytometry. Gelatin zymograms showed that the degradative activity of 72-kD gelatinases was greater in L5 cells than P cells. These results indicate that, in addition to adhesiveness and motility, the invasive ability of L5 cells may also be attributed to enhanced gelatinolytic activity. L5 cells grew more rapidly than P cells in vitro. Thus, an experimental model using highly metastatic colon 26 L5 cells would be useful for analyzing the molecular mechanism of liver metastasis and for evaluating the efficacy of treatment of occult micrometastases which may already have been disseminated at the time of surgery. |
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Authors:
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Y Ohnishi; T Sakamoto; H Fujii; F Kimura; J Murata; K Tazawa; M Fujimaki; Y Sato; M Kondo; Y Une; J Uchino; I Saiki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine Volume: 18 ISSN: 1010-4283 ISO Abbreviation: Tumour Biol. Publication Date: 1997 |
Date Detail:
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Created Date: 1997-08-05 Completed Date: 1997-08-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8409922 Medline TA: Tumour Biol Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 113-22 Citation Subset: IM |
Affiliation:
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Department of Pathogenic Biochemistry, Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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pathology,
secondary* Animals Cell Adhesion Cell Movement Colonic Neoplasms / pathology* Female Integrin alpha4beta1 Integrins / physiology Liver Neoplasms / secondary* Mice Mice, Inbred BALB C Neoplasm Invasiveness Receptors, Collagen Receptors, Lymphocyte Homing / physiology Specific Pathogen-Free Organisms |
| Chemical | |
Reg. No./Substance:
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0/Integrin alpha4beta1; 0/Integrins; 0/Receptors, Collagen; 0/Receptors, Lymphocyte Homing |
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