Document Detail

Characterization of lipopolysaccharides from Escherichia coli K-12 mutants.
MedLine Citation:
PMID:  1089628     Owner:  NLM     Status:  MEDLINE    
Chemical analyses of the carbohydrate composition of lipopolysaccharides (LPS) from a number of LPS mutants were used to propose a schematic composition for the LPS from Escherichia coli K-12. The formula contains four regions: the first consists of lipid A, ketodeoxyoctonoic acid, and a phosphorous component; the second contains only heptose; the third only glucose; and the fourth additional glucose, galactose, and rhamnose. LPS from E. coli B may have a similar composition but lacks the galactose and rhamnose units. A set of LPS-specific bacteriophages were used for comparing three mutants of Salmonella with a number of LPS mutants of E. coli K-12. The results confirm that there are basic similarities in the first and second regions of the LPS structure; they also support the four region divisions of the LPS formula. Paper chromatography was used for characterization of 32-P-labeled LPS from different strains of E. coli and Salmonella. The Rf values for LPS varied from 0.27 to 0.75 depending on the amounts of carbohydrates in the molecule. LPS from all strains studied was homogenous except for strain D31 which produced two types of LPS. Mild acid hydrolysis of labeled LPS liberated lipid A and two other components with phosphate, one of which was assigned to the first region. It is suggested that paper chromatography can be used in biosynthetic studies concerning regions 2 to 4.
H G Boman; D A Monner
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  121     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1975 Feb 
Date Detail:
Created Date:  1975-05-10     Completed Date:  1975-05-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  455-64     Citation Subset:  IM    
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MeSH Terms
Carbohydrates / analysis
Chromatography, Gas
Chromatography, Paper
DNA Viruses
Escherichia coli / analysis*
Fatty Acids / analysis
Galactose / analysis
Glucosamine / analysis
Glucose / analysis
Heptoses / analysis
Lipopolysaccharides / analysis*
Phosphates / analysis
Phosphorus Radioisotopes
Polysaccharides, Bacterial / analysis*
Rhamnose / analysis
Ribose / analysis
Salmonella / analysis
Reg. No./Substance:
0/Carbohydrates; 0/Fatty Acids; 0/Heptoses; 0/Lipopolysaccharides; 0/Phosphates; 0/Phosphorus Radioisotopes; 0/Polysaccharides, Bacterial; 10485-94-6/Rhamnose; 26566-61-0/Galactose; 3416-24-8/Glucosamine; 50-69-1/Ribose; 50-99-7/Glucose

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