| Characterization of the lipopolysaccharide and structure of the O-specific polysaccharide of the bacterium Pseudomonas syringae pv. atrofaciens IMV 948. | |
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MedLine Citation:
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PMID: 11403642 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipopolysaccharide (LPS) was isolated from the phytopathogenic bacterium Pseudomonas syringae pv. atrofaciens IMV 948 by mild extraction of the microbial cells with saline, and the properties, composition, and structure of the LPS were studied. The LPS showed low toxicity in D- galactosamine-sensitized mice and low biological activity in plants. Structural components of LPS--lipid A, core oligosaccharide, and O-specific polysaccharide (OPS)--were obtained by mild acid degradation and characterized. The lipid A contained fatty acids 3-HO-C10:0, C12:0, 2-HO-C12:0, 3-HO-C12:0, C16:0, C16:1, C18:0, and C18:1, as well as components of the hydrophilic moiety: GlcN, ethanolamine, phosphate, and phosphoethanolamine. The LPS core contained components typical of pseudomonads: glucose, rhamnose (Rha), L-glycero-D-manno-heptose, GlcN, GalN, 2-keto-3-deoxy-D-manno-octonic acid, alanine, and phosphate. The OPS consisted of L-Rha and D-GlcNAc in the ratio 4 : 1 and was structurally heterogeneous. The main pentasaccharide repeating unit of the OPS has the following structure: [structure see text]. Immunochemical studies showed that P. syringae pv. atrofaciens IMV 948 is serologically separate from other P. syringae strains, including those that have structurally similar OPS. |
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Authors:
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G M Zdorovenko; A S Shashkov; E L Zdorovenko; N A Kocharova; L M Yakovleva; Y A Knirel; K Rudolph |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry. Biokhimii͡a Volume: 66 ISSN: 0006-2979 ISO Abbreviation: Biochemistry Mosc. Publication Date: 2001 Apr |
Date Detail:
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Created Date: 2001-06-13 Completed Date: 2001-07-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376536 Medline TA: Biochemistry (Mosc) Country: United States |
Other Details:
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Languages: eng Pagination: 369-77 Citation Subset: IM |
Affiliation:
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Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, 252143, Ukraine. zdorov@i.kiev.ua |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Gel
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methods Enzyme-Linked Immunosorbent Assay / methods Immunoelectrophoresis / methods Lipid A / analysis*, chemistry, isolation & purification Lipopolysaccharides / analysis*, chemistry*, isolation & purification Magnetic Resonance Spectroscopy / methods O Antigens / analysis*, chemistry*, isolation & purification Pseudomonas / chemistry*, classification Serotyping |
| Chemical | |
Reg. No./Substance:
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0/Lipid A; 0/Lipopolysaccharides; 0/O Antigens |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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