Document Detail


Characterization of the lipopolysaccharide and structure of the O-specific polysaccharide of the bacterium Pseudomonas syringae pv. atrofaciens IMV 948.
MedLine Citation:
PMID:  11403642     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipopolysaccharide (LPS) was isolated from the phytopathogenic bacterium Pseudomonas syringae pv. atrofaciens IMV 948 by mild extraction of the microbial cells with saline, and the properties, composition, and structure of the LPS were studied. The LPS showed low toxicity in D- galactosamine-sensitized mice and low biological activity in plants. Structural components of LPS--lipid A, core oligosaccharide, and O-specific polysaccharide (OPS)--were obtained by mild acid degradation and characterized. The lipid A contained fatty acids 3-HO-C10:0, C12:0, 2-HO-C12:0, 3-HO-C12:0, C16:0, C16:1, C18:0, and C18:1, as well as components of the hydrophilic moiety: GlcN, ethanolamine, phosphate, and phosphoethanolamine. The LPS core contained components typical of pseudomonads: glucose, rhamnose (Rha), L-glycero-D-manno-heptose, GlcN, GalN, 2-keto-3-deoxy-D-manno-octonic acid, alanine, and phosphate. The OPS consisted of L-Rha and D-GlcNAc in the ratio 4 : 1 and was structurally heterogeneous. The main pentasaccharide repeating unit of the OPS has the following structure: [structure see text]. Immunochemical studies showed that P. syringae pv. atrofaciens IMV 948 is serologically separate from other P. syringae strains, including those that have structurally similar OPS.
Authors:
G M Zdorovenko; A S Shashkov; E L Zdorovenko; N A Kocharova; L M Yakovleva; Y A Knirel; K Rudolph
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry. Biokhimii͡a     Volume:  66     ISSN:  0006-2979     ISO Abbreviation:  Biochemistry Mosc.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-06-13     Completed Date:  2001-07-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376536     Medline TA:  Biochemistry (Mosc)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  369-77     Citation Subset:  IM    
Affiliation:
Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, 252143, Ukraine. zdorov@i.kiev.ua
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Gel / methods
Enzyme-Linked Immunosorbent Assay / methods
Immunoelectrophoresis / methods
Lipid A / analysis*,  chemistry,  isolation & purification
Lipopolysaccharides / analysis*,  chemistry*,  isolation & purification
Magnetic Resonance Spectroscopy / methods
O Antigens / analysis*,  chemistry*,  isolation & purification
Pseudomonas / chemistry*,  classification
Serotyping
Chemical
Reg. No./Substance:
0/Lipid A; 0/Lipopolysaccharides; 0/O Antigens

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