Document Detail


Characterization of leukemic cell behaviors in a soft marrow mimetic alginate hydrogel.
MedLine Citation:
PMID:  22888018     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alginate hydrogels possess tunable mechanical properties that can mimic soft marrow tissue and present three-dimensional (3D) cues. This study evaluates its utility for supporting leukemic cell growth in vitro and its impact on cell survival, growth, and differentiation. Our results showed that the standard viscosity alginates had compromised leukemia cell viability but lower viscosity alginates recovered cell viability and improved 3D cell proliferation (27 fold) compared to 2D cultures (18 fold). Conjugation with RGD peptides promoted further cell growth (43 folds). In general, 3D hydrogels supported high-density cultures better than 2D cultures. Leukemic cells formed densely packed cell clusters in alginate hydrogels and spontaneously differentiated into a more diverse myeloid population. The cell cycle data suggested that more cells go into active cycling with a G2/M arrest in alginate hydrogels and the presence of multiploidy confirmed maturation toward megakaryocytes. In summary, superior culture of leukemia cells in 3D hydrogels is demonstrated in this study accompanied by a potential role of physical cues influencing cell fate decision. Manipulation of biophysical and biochemical properties of alginate hydrogels permits the study of specific interactions and serves to provide a robust 3D platform for studying extrinsic contributions inside the bone marrow.
Authors:
Thao Thi Thu Vu; Carine Lim; Mayasari Lim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-10
Journal Detail:
Title:  Journal of biomedical materials research. Part B, Applied biomaterials     Volume:  100     ISSN:  1552-4981     ISO Abbreviation:  J. Biomed. Mater. Res. Part B Appl. Biomater.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-05     Completed Date:  2013-01-15     Revised Date:  2013-06-10    
Medline Journal Info:
Nlm Unique ID:  101234238     Medline TA:  J Biomed Mater Res B Appl Biomater     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1980-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
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MeSH Terms
Descriptor/Qualifier:
Alginates / pharmacology*
Biomimetic Materials / pharmacology*
Bone Marrow*
Cell Survival / drug effects
G2 Phase Cell Cycle Checkpoints / drug effects*
Glucuronic Acid / pharmacology
Hexuronic Acids / pharmacology
Humans
K562 Cells
Leukemia / metabolism*,  pathology
M Phase Cell Cycle Checkpoints / drug effects*
Megakaryocytes / metabolism,  pathology
Polyploidy
Chemical
Reg. No./Substance:
0/Alginates; 0/Hexuronic Acids; 576-37-4/Glucuronic Acid; 9005-32-7/alginic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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