| Characterization of a lectin from goat peripheral blood lymphocytes. | |
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MedLine Citation:
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PMID: 9219437 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A D-glucose specific lectin was isolated from goat peripheral blood lymphocytes by affinity chromatography on N-acetyl D-glucosamine agarose gel. The fluorescence intensity of 4 methyl umbelliferyl D-glucose was quenched to about 62% on addition of the lectin. This lectin gave a single band corresponding to 112 kDa in SDS-PAGE irrespective of treatment with 2-mercaptoethanol. The molecular weight and the Stoke's radius of the lectin in the native conditions were found to be 114 kDa and 4.54 nm, respectively, as determined by gel filtration on Sephacryl S 500 column. The lectin was found to be a glycoprotein with 5.6% of neutral hexose content and 5.5% of sialic acid. The lectin agglutinated trypsinized rabbit erythrocytes and human type A erythrocytes. The hemagglutinating activity was dependent on the presence of divalent cations like Mn2+ and Ca2+. Optimum pH, ionic strength and temperature for rebinding of lectin to acid treated Sephadex G200 were found to be 7.5, 0.16 and 30-37 degrees C, respectively. |
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Authors:
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R Kayestha; Sumati; K Hajela |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Indian journal of biochemistry & biophysics Volume: 33 ISSN: 0301-1208 ISO Abbreviation: Indian J. Biochem. Biophys. Publication Date: 1996 Dec |
Date Detail:
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Created Date: 1997-08-05 Completed Date: 1997-08-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0310774 Medline TA: Indian J Biochem Biophys Country: INDIA |
Other Details:
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Languages: eng Pagination: 506-11 Citation Subset: IM |
Affiliation:
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School of Life Sciences, Devi Ahilya Vishwavidyalaya, Indore, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Goats / blood* Hemagglutination Hydrogen-Ion Concentration Lectins / chemistry*, isolation & purification, metabolism, pharmacology Lymphocytes / chemistry* Molecular Weight Osmolar Concentration Protein Binding Spectrometry, Fluorescence Temperature |
| Chemical | |
Reg. No./Substance:
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0/Lectins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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