Document Detail


Characterization of a lectin from goat peripheral blood lymphocytes.
MedLine Citation:
PMID:  9219437     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A D-glucose specific lectin was isolated from goat peripheral blood lymphocytes by affinity chromatography on N-acetyl D-glucosamine agarose gel. The fluorescence intensity of 4 methyl umbelliferyl D-glucose was quenched to about 62% on addition of the lectin. This lectin gave a single band corresponding to 112 kDa in SDS-PAGE irrespective of treatment with 2-mercaptoethanol. The molecular weight and the Stoke's radius of the lectin in the native conditions were found to be 114 kDa and 4.54 nm, respectively, as determined by gel filtration on Sephacryl S 500 column. The lectin was found to be a glycoprotein with 5.6% of neutral hexose content and 5.5% of sialic acid. The lectin agglutinated trypsinized rabbit erythrocytes and human type A erythrocytes. The hemagglutinating activity was dependent on the presence of divalent cations like Mn2+ and Ca2+. Optimum pH, ionic strength and temperature for rebinding of lectin to acid treated Sephadex G200 were found to be 7.5, 0.16 and 30-37 degrees C, respectively.
Authors:
R Kayestha; Sumati; K Hajela
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Indian journal of biochemistry & biophysics     Volume:  33     ISSN:  0301-1208     ISO Abbreviation:  Indian J. Biochem. Biophys.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-08-05     Completed Date:  1997-08-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0310774     Medline TA:  Indian J Biochem Biophys     Country:  INDIA    
Other Details:
Languages:  eng     Pagination:  506-11     Citation Subset:  IM    
Affiliation:
School of Life Sciences, Devi Ahilya Vishwavidyalaya, Indore, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Goats / blood*
Hemagglutination
Hydrogen-Ion Concentration
Lectins / chemistry*,  isolation & purification,  metabolism,  pharmacology
Lymphocytes / chemistry*
Molecular Weight
Osmolar Concentration
Protein Binding
Spectrometry, Fluorescence
Temperature
Chemical
Reg. No./Substance:
0/Lectins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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