Document Detail

Characterization of label-retaining cells in the epidermis of a human skin equivalent.
MedLine Citation:
PMID:  9146730     Owner:  NLM     Status:  MEDLINE    
The human skin equivalent (HSE) is an in vitro reconstructed model that resembles skin morphologically and biochemically. The HSE is formed by overlaying a fibroblast-populated collagen matrix with a suspension of epidermal cells. Basal keratinocytes attach to the dermal equivalent via a newly formed basement membrane and multiply to form a stratified, differentiated epidermis. The aim of the studies described here was to characterize the basal cells of the HSE in terms of their cell cycling potential. The experiments utilized long-term labelling of the cells with tritiated thymidine ([3H]dT), followed by irradiation with ultraviolet light. [3H]dT incorporation was analysed via routine autoradiography. Irradiation with 100 J/m2 UV light increased the number of labelled basal cells by 58% over the control, the maximal stimulation observed. Decreased numbers of labelled basal cells were observed at doses of UV light greater than 100 J/m2. The maximal number of labelled basal cells was observed on day 14 and decreased over time; the number of labelled suprabasal cells increased concomitantly. Label-retaining cells (12%) persisted in the stratum basale of control HSEs after 32 days in culture. Labelled cells were observed in the apical layers of the stratum granulosum of control HSEs after 22 days in culture. These data suggest that the stratum basale of the HSE contains a population of slow-cycling cells whose characteristics resemble a subpopulation of slowly cycling cells found in normal human skin.
L J Graeter; B E Hull
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell proliferation     Volume:  29     ISSN:  0960-7722     ISO Abbreviation:  Cell Prolif.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-06-04     Completed Date:  1997-06-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  679-88     Citation Subset:  IM    
Biomedical Sciences PhD Program, Wright State University, Dayton, Ohio, USA.
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MeSH Terms
Cell Cycle
Cells, Cultured
Epidermis / cytology*,  radiation effects
Skin, Artificial*
Ultraviolet Rays
Grant Support

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