Document Detail


Characterization and kinetic analysis of enzyme-substrate recognition by three recombinant lactococcal tripeptidases.
MedLine Citation:
PMID:  15752689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tripeptidases from Lactococcus lactis subsp. lactis (L9PepTR), L. lactis subsp. cremoris (L6PepTR), and L. lactis subsp. hordniae (hTPepTR) were cloned, overexpressed, purified, and characterized. Although these enzymes contained three to seven naturally occurring amino acid differences, both metal-binding and catalytic sites were highly conserved. The k(cat) values of hTPepTR were approximately 1.5- to 2-fold higher than those of L9PepTR, while, for L6PepTR, they were approximately 0.8- to 1.4-times the L9PepTR values. The K(m) of tripeptidase from subsp. lactis (L9PepTR) was considerably larger when glycine was the amino acid located at both the N- and C-terminus of the peptide substrate. In addition, the K(m) values of L9PepTR increased in the following order for YGG, LGG, FGG, SGG, and alpha-aminoisobutyrylglycylglycine, while the k(cat)/K(m) decreased in the same order. These results suggest that the dipole moment and steric hindrance of the N-terminal amino acid side chain may be the most important factors controlling substrate specificity.
Authors:
Sumiko Mori; Satoru Nirasawa; Shiro Komba; Takafumi Kasumi
Related Documents :
166659 - The catalytic mechanism of amino acid:trna ligases. synergism and formation of the tern...
4037309 - Assay of human fibrinopeptides by high-performance liquid chromatography.
10415099 - Preparation of insoluble transmembrane peptides: glycophorin-a, prion (110-137), and fg...
3295239 - Renin inhibitors. design of angiotensinogen transition-state analogues containing novel...
18969349 - Influence of amide linkages on acidity determinations of humic substances testing with ...
7783229 - Evolution of the aminoacyl-trna synthetases and the origin of the genetic code.
16583309 - High concentrations of d-amino acids in human gastric juice.
6428369 - Isonicotinic acid hydrazide induced changes and inhibition in mycolic acid synthesis in...
3032669 - A major metabolite of delta 1-tetrahydrocannabinol reduces its cataleptic effect in mice.
Publication Detail:
Type:  Journal Article     Date:  2005-01-21
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1748     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-08     Completed Date:  2005-04-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  26-34     Citation Subset:  IM    
Affiliation:
National Food Research Institute, Tsukuba, Ibaraki 305-8642, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Aminopeptidases / chemistry,  genetics,  metabolism*
Bacterial Proteins / chemistry,  genetics,  metabolism*
Hydrogen-Ion Concentration
Lactococcus lactis / enzymology*
Models, Molecular
Molecular Sequence Data
Protein Structure, Tertiary
Recombinant Proteins / chemistry,  genetics,  metabolism*
Sequence Alignment
Substrate Specificity
Temperature
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Recombinant Proteins; EC 3.4.11.-/Aminopeptidases; EC 3.4.11.14/PepT tripeptidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Changing the metal ion selectivity of rabbit muscle enolase by mutagenesis: effects of the G37A and ...
Next Document:  Cloning and expression of ostrich trypsinogen: an avian trypsin with a highly sensitive autolysis si...