| Characterization of kidney epithelial cells from the Florida manatee, Trichechus manatus latirostris. | |
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MedLine Citation:
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PMID: 11515973 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The West-Indian manatee, Trichechus manatus latirostris, is a herbivorous marine mammal found in the coastal waters of Florida. Because of their endangered status, animal experimentation is not allowed. Therefore, a cell line was developed and characterized from tissue collected during necropsies of the manatees. A primary cell culture was established by isolating single cells from kidney tissue using both enzymatic and mechanical techniques. Primary manatee kidney (MK) cells were subcultured for characterization. These cells were morphologically similar to the cell lines of epithelial origin. An immunocytochemistry assay was used to localize the cytokeratin filaments common to cells of epithelial origin. At second passage, epithelial-like cells had an average population-doubling time of 48 h, had an optimum seeding density of 5 x 10(3) cells/cm2, and readily attached to plastic culture plates with a high level of seeding efficiency. Although the epithelial-like cells had a rapid growth rate during the first three passages, the cloning potential was low. These cells did not form colonies in agar medium, were serum dependent, had a limited life span of approximately nine passages, and possessed cell-contact inhibition. These data suggest that the cells were finite (noncontinuous growth), did not possess transformed properties, and were of epithelial origin. These cells are now referred to as MK epithelial cells. |
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Authors:
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D D Sweat JMDunigan; S D Wright |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: In vitro cellular & developmental biology. Animal Volume: 37 ISSN: 1071-2690 ISO Abbreviation: In Vitro Cell. Dev. Biol. Anim. Publication Date: 2001 Jun |
Date Detail:
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Created Date: 2001-08-22 Completed Date: 2002-01-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9418515 Medline TA: In Vitro Cell Dev Biol Anim Country: United States |
Other Details:
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Languages: eng Pagination: 386-94 Citation Subset: IM |
Affiliation:
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Marine Mammal Pathobiology Laboratory, Florida Fish and Wildlife Conservation Commission, St. Petersburg 33711, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Culture Techniques / methods* Cell Survival Cells, Cultured Epithelial Cells / cytology, metabolism Fibroblasts / cytology Florida Humans Keratins / metabolism Kidney / cytology* Trichechus manatus* |
| Chemical | |
Reg. No./Substance:
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68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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