| Characterization of kallikrein-related peptidase 4 glycosylations. | |
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MedLine Citation:
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PMID: 22243251 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Yamakoshi Y, Yamakoshi F, Hu JC-C, Simmer JP. Characterization of kallikrein-related peptidase 4 glycosylations. Eur J Oral Sci 2011; 119 (Suppl. 1): 234-240. © 2011 Eur J Oral Sci Kallikrein-related peptidase 4 (KLK4) is a glycosylated serine protease that functions in the maturation (hardening) of dental enamel. Pig and mouse KLK4 contain three potential N-glycosylation sites. We isolated KLK4 from developing pig and mouse molars and characterized their N-glycosylations. N-glycans were enzymatically released by digestion with N-glycosidase F and fluorescently labeled with 2-aminobenzoic acid. Normal-phase high-performance liquid chromatography (NP-HPLC) revealed N-glycans with no, or with one, two, or three sialic acid attachments in pig KLK4 and with no, or with one or two sialic acid attachments in mouse KLK4. The labeled N-glycans were digested with sialidase to generate the asialo N-glycan cores that were fractionated by reverse-phase HPLC, and their retention times were compared with similarly labeled glycan standards. The purified cores were characterized by mass spectrometric and monosaccharide composition analyses. We determined that pig and mouse KLK4 have NA2 and NA2F biantennary N-glycan cores. The pig triantennary core is NA3. The mouse triantennary core is NA3 with a fucose connected by an α1-6 linkage, indicating that it is attached to the first N-acetyglucosamine (NA3F). We conclude that pig KLK4 has NA2, NA2F, and NA3 N-glycan cores with no, or with one, two, or three sialic acids. Mouse KLK4 has NA2, NA2F, and NA3F N-glycan cores with no, or with one or two sialic acids. |
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Authors:
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Yasuo Yamakoshi; Fumiko Yamakoshi; Jan C-C Hu; James P Simmer |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European journal of oral sciences Volume: 119 Suppl 1 ISSN: 1600-0722 ISO Abbreviation: Eur. J. Oral Sci. Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2012-01-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9504563 Medline TA: Eur J Oral Sci Country: Denmark |
Other Details:
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Languages: eng Pagination: 234-40 Citation Subset: D; IM |
Copyright Information:
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© 2011 Eur J Oral Sci. |
Affiliation:
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Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Eisenhower Place, Ann Arbor, MI, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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