| Characterization of kallikrein-related peptidase 4 glycosylations. | |
| | |
MedLine Citation:
|
PMID: 22243251 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Kallikrein-related peptidase 4 (KLK4) is a glycosylated serine protease that functions in the maturation (hardening) of dental enamel. Pig and mouse KLK4 contain three potential N-glycosylation sites. We isolated KLK4 from developing pig and mouse molars and characterized their N-glycosylations. N-glycans were enzymatically released by digestion with N-glycosidase F and fluorescently labeled with 2-aminobenzoic acid. Normal-phase high-performance liquid chromatography (NP-HPLC) revealed N-glycans with no, or with one, two, or three sialic acid attachments in pig KLK4 and with no, or with one or two sialic acid attachments in mouse KLK4. The labeled N-glycans were digested with sialidase to generate the asialo N-glycan cores that were fractionated by reverse-phase HPLC, and their retention times were compared with similarly labeled glycan standards. The purified cores were characterized by mass spectrometric and monosaccharide composition analyses. We determined that pig and mouse KLK4 have NA2 and NA2F biantennary N-glycan cores. The pig triantennary core is NA3. The mouse triantennary core is NA3 with a fucose connected by an α1-6 linkage, indicating that it is attached to the first N-acetyglucosamine (NA3F). We conclude that pig KLK4 has NA2, NA2F, and NA3 N-glycan cores with no, or with one, two, or three sialic acids. Mouse KLK4 has NA2, NA2F, and NA3F N-glycan cores with no, or with one or two sialic acids. |
| | |
Authors:
|
Yasuo Yamakoshi; Fumiko Yamakoshi; Jan C-C Hu; James P Simmer |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: European journal of oral sciences Volume: 119 Suppl 1 ISSN: 1600-0722 ISO Abbreviation: Eur. J. Oral Sci. Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2012-01-16 Completed Date: 2012-06-18 Revised Date: 2013-02-19 |
Medline Journal Info:
|
Nlm Unique ID: 9504563 Medline TA: Eur J Oral Sci Country: Denmark |
Other Details:
|
Languages: eng Pagination: 234-40 Citation Subset: D; IM |
Copyright Information:
|
© 2011 Eur J Oral Sci. |
Affiliation:
|
Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Eisenhower Place, Ann Arbor, MI 48108, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Chromatography, High Pressure Liquid / methods Dental Enamel Proteins / metabolism* Glycosylation Humans Kallikreins / analysis, chemistry*, metabolism* Mice Molecular Structure N-Acetylneuraminic Acid / chemistry Polysaccharides / chemistry Protein Processing, Post-Translational* Recombinant Proteins / chemistry, metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sus scrofa |
| Grant Support | |
ID/Acronym/Agency:
|
DE015846/DE/NIDCR NIH HHS; DE019775/DE/NIDCR NIH HHS; R01 DE015846-09/DE/NIDCR NIH HHS; R01 DE019775/DE/NIDCR NIH HHS; R01 DE019775-04/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Dental Enamel Proteins; 0/Polysaccharides; 0/Recombinant Proteins; 131-48-6/N-Acetylneuraminic Acid; EC 3.4.21.-/Kallikreins; EC 3.4.21.-/kallikrein 4 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Expression of kallikrein-related peptidase 4 in dental and non-dental tissues.
Next Document: Molecular evolution of matrix metalloproteinase 20.