Document Detail


Characterization of kallikrein-related peptidase 4 glycosylations.
MedLine Citation:
PMID:  22243251     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Kallikrein-related peptidase 4 (KLK4) is a glycosylated serine protease that functions in the maturation (hardening) of dental enamel. Pig and mouse KLK4 contain three potential N-glycosylation sites. We isolated KLK4 from developing pig and mouse molars and characterized their N-glycosylations. N-glycans were enzymatically released by digestion with N-glycosidase F and fluorescently labeled with 2-aminobenzoic acid. Normal-phase high-performance liquid chromatography (NP-HPLC) revealed N-glycans with no, or with one, two, or three sialic acid attachments in pig KLK4 and with no, or with one or two sialic acid attachments in mouse KLK4. The labeled N-glycans were digested with sialidase to generate the asialo N-glycan cores that were fractionated by reverse-phase HPLC, and their retention times were compared with similarly labeled glycan standards. The purified cores were characterized by mass spectrometric and monosaccharide composition analyses. We determined that pig and mouse KLK4 have NA2 and NA2F biantennary N-glycan cores. The pig triantennary core is NA3. The mouse triantennary core is NA3 with a fucose connected by an α1-6 linkage, indicating that it is attached to the first N-acetyglucosamine (NA3F). We conclude that pig KLK4 has NA2, NA2F, and NA3 N-glycan cores with no, or with one, two, or three sialic acids. Mouse KLK4 has NA2, NA2F, and NA3F N-glycan cores with no, or with one or two sialic acids.
Authors:
Yasuo Yamakoshi; Fumiko Yamakoshi; Jan C-C Hu; James P Simmer
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  European journal of oral sciences     Volume:  119 Suppl 1     ISSN:  1600-0722     ISO Abbreviation:  Eur. J. Oral Sci.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2012-01-16     Completed Date:  2012-06-18     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  9504563     Medline TA:  Eur J Oral Sci     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  234-40     Citation Subset:  D; IM    
Copyright Information:
© 2011 Eur J Oral Sci.
Affiliation:
Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Eisenhower Place, Ann Arbor, MI 48108, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Chromatography, High Pressure Liquid / methods
Dental Enamel Proteins / metabolism*
Glycosylation
Humans
Kallikreins / analysis,  chemistry*,  metabolism*
Mice
Molecular Structure
N-Acetylneuraminic Acid / chemistry
Polysaccharides / chemistry
Protein Processing, Post-Translational*
Recombinant Proteins / chemistry,  metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Sus scrofa
Grant Support
ID/Acronym/Agency:
DE015846/DE/NIDCR NIH HHS; DE019775/DE/NIDCR NIH HHS; R01 DE015846-09/DE/NIDCR NIH HHS; R01 DE019775/DE/NIDCR NIH HHS; R01 DE019775-04/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Dental Enamel Proteins; 0/Polysaccharides; 0/Recombinant Proteins; 131-48-6/N-Acetylneuraminic Acid; EC 3.4.21.-/Kallikreins; EC 3.4.21.-/kallikrein 4
Comments/Corrections

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