Document Detail

Characterization of C/EBPbeta isoforms in normal versus neoplastic mammary epithelial cells.
MedLine Citation:
PMID:  11573208     Owner:  NLM     Status:  MEDLINE    
A member of the CCAAT Enhancer Binding Proteins (C/EBPs) family of transcription factors, C/EBPbeta, has recently proven to be an important player in both growth and differentiation of the epithelial cells in the mammary gland. When the gene for C/EBPbeta is disrupted in mice, these mice fail to either develop normal mammary ducts during puberty or pregnancy, or to lactate upon parturition. C/EBPbeta can be present in cells in three isoforms: C/EBPbeta-1, -2, and -3. These isoforms have the same carboxy terminus but different N-termini due to alternative translational initiation at three different initiator codons within the C/EBPbeta mRNA. Using a commercially available antibody specific to the C-terminus of C/EBPbeta and a novel antibody specific to the N-terminus of C/EBPbeta-1, we have uncovered a striking difference in the forms of C/EBPbeta present in normal mammary epithelial cells versus breast cancer cell lines. C/EBPbeta- 1 is found exclusively in normal mammary epithelial cells, whereas C/EBPbeta- 2 is found only in dividing cells, both normal and neoplastic. Our preliminary data suggest that the prevalent form of C/EBPbeta in cancer cells, C/EBPbeta- 2, can activate genes which push the cell to divide, such as cyclin D1.
E M Eaton; M Hanlon; L Bundy; L Sealy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  189     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-09-26     Completed Date:  2001-10-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  91-105     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Wiley-Liss, Inc.
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.
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MeSH Terms
Antibodies / immunology
Breast / cytology,  metabolism*
Breast Neoplasms / metabolism*
CCAAT-Enhancer-Binding Protein-beta / immunology,  metabolism*,  physiology*
Carcinoma / metabolism*
Cell Nucleus / metabolism
Cells, Cultured
Cyclin D1 / genetics
Epithelial Cells / metabolism
Promoter Regions, Genetic
Protein Isoforms / immunology,  metabolism,  physiology
Tumor Cells, Cultured
Grant Support
2T32DK07563-13/DK/NIDDK NIH HHS; 2T32GM08554-06/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Antibodies; 0/CCAAT-Enhancer-Binding Protein-beta; 0/Protein Isoforms; 136601-57-5/Cyclin D1
Erratum In:
J Cell Physiol 2002 Jan;190(1):131

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