| Characterization of C/EBPbeta isoforms in normal versus neoplastic mammary epithelial cells. | |
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MedLine Citation:
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PMID: 11573208 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A member of the CCAAT Enhancer Binding Proteins (C/EBPs) family of transcription factors, C/EBPbeta, has recently proven to be an important player in both growth and differentiation of the epithelial cells in the mammary gland. When the gene for C/EBPbeta is disrupted in mice, these mice fail to either develop normal mammary ducts during puberty or pregnancy, or to lactate upon parturition. C/EBPbeta can be present in cells in three isoforms: C/EBPbeta-1, -2, and -3. These isoforms have the same carboxy terminus but different N-termini due to alternative translational initiation at three different initiator codons within the C/EBPbeta mRNA. Using a commercially available antibody specific to the C-terminus of C/EBPbeta and a novel antibody specific to the N-terminus of C/EBPbeta-1, we have uncovered a striking difference in the forms of C/EBPbeta present in normal mammary epithelial cells versus breast cancer cell lines. C/EBPbeta- 1 is found exclusively in normal mammary epithelial cells, whereas C/EBPbeta- 2 is found only in dividing cells, both normal and neoplastic. Our preliminary data suggest that the prevalent form of C/EBPbeta in cancer cells, C/EBPbeta- 2, can activate genes which push the cell to divide, such as cyclin D1. |
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Authors:
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E M Eaton; M Hanlon; L Bundy; L Sealy |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular physiology Volume: 189 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 2001 Oct |
Date Detail:
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Created Date: 2001-09-26 Completed Date: 2001-10-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 91-105 Citation Subset: IM |
Copyright Information:
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Copyright 2001 Wiley-Liss, Inc. |
Affiliation:
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Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies
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immunology Breast / cytology, metabolism* Breast Neoplasms / metabolism* CCAAT-Enhancer-Binding Protein-beta / immunology, metabolism*, physiology* Carcinoma / metabolism* Cell Nucleus / metabolism Cells, Cultured Cyclin D1 / genetics Epithelial Cells / metabolism Female Humans Promoter Regions, Genetic Protein Isoforms / immunology, metabolism, physiology Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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2T32DK07563-13/DK/NIDDK NIH HHS; 2T32GM08554-06/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/CCAAT-Enhancer-Binding Protein-beta; 0/Protein Isoforms; 136601-57-5/Cyclin D1 |
| Comments/Corrections | |
Erratum In:
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J Cell Physiol 2002 Jan;190(1):131 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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