Document Detail


Characterization of ion transport in the isolated epipodite of the lobster Homarus americanus.
MedLine Citation:
PMID:  20086126     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Unfolded epipodite isolated from American lobsters (Homarus americanus) acclimated to dilute seawater was mounted in an Ussing-type chamber for ion transport studies. The split epipodite is an electrically polarized, one-cell-layer epithelium supported with cuticle. Under open-circuit conditions, the transepithelial potential was -4.2+/-1.0 mV (N=38). In the short-circuited epithelium, the current averaged over all of the preparations was -185.4+/-20.2 A cm(-2) (N=38) with a high conductance of 55.2+/-11.4 mS cm(-2) (N=38), typical for a leaky epithelium. The Na:Cl absorptive flux ratio was 1:1.6; ion substitution experiments indicated that the transport of Na+ and Cl- is coupled. Basolateral application of the Cl- channel blockers 5-nitro-2-(3-phenylpropylamino) benzoate (NPPB) and niflumic acid (NFA) dose-dependently inhibited short-circuit current (ISC). Secretory K+ (Rb+) fluxes exceeded influxes and were inhibited by the Na+/K+-ATPase inhibitor ouabain and the K+ channel blocker cesium. Western blot analysis showed that Na+/K+-ATPase alpha-subunit protein was more highly expressed in the epipodite of lobsters acclimated to 20 p.p.t. compared with animals acclimated to seawater (34 p.p.t.). 3-Isobutyl-1-methyl-xanthine (IBMX) stimulated a negative ISC and enhanced apical secretory K+ flux. Basolateral application of NPPB inhibited JRbB-->A fluxes, suggesting the interaction of K+ channels with NPPB-sensitive Cl- channels. The results are summarized in a transport model, suggesting apical Na+/K+/2Cl- co-transport, a dominant apical K+-secreting channel and basolaterally located Cl- and K+ channels. This study represents the first comprehensive characterization of ion transport processes across the lobster epipodite epithelium and indeed in any tissue within the branchial cavity of the American lobster.
Authors:
C Lucu; D W Towle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  213     ISSN:  1477-9145     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-20     Completed Date:  2010-03-16     Revised Date:  2010-05-19    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  418-25     Citation Subset:  IM    
Affiliation:
Mount Desert Island Biological Laboratory, Salisbury Cove, ME 04672, USA. cedomil.lucu@unidu.hr
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MeSH Terms
Descriptor/Qualifier:
Animal Structures / metabolism*
Animals
Blotting, Western
Chlorides / metabolism
Electrolytes / metabolism
Ion Channel Gating / physiology
Ion Transport
Models, Biological
Nephropidae / anatomy & histology*,  metabolism*
Potassium / metabolism
Sodium / metabolism
Time Factors
Chemical
Reg. No./Substance:
0/Chlorides; 0/Electrolytes; 7440-09-7/Potassium; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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