Document Detail

Characterization of the interface between adsorbed fibronectin and human embryonic stem cells.
MedLine Citation:
PMID:  23554347     Owner:  NLM     Status:  MEDLINE    
The cell-substrate interface plays a key role in the regulation of cell behaviour. Defining the properties of this interface is particularly important for human embryonic stem (hES) cell culture, because changes in this environment can regulate hES cell differentiation. It has been established that fibronectin-coated surfaces can promote the attachment, growth and maintenance of the undifferentiated phenotype of hES cells. We investigated the influence of the surface density of adsorbed fibronectin on hES cell behaviour in defined serum-free culture conditions and demonstrated that only 25 per cent surface saturation was required to maintain attachment, growth and maintenance of the undifferentiated phenotype. The influence of surface-adsorbed fibronectin fragments was compared with whole fibronectin, and it was demonstrated that the 120 kDa fragment central binding domain alone was able to sustain hES cells in an undifferentiated phenotype in a similar fashion to fibronectin. Furthermore, hES cell attachment to both fibronectin and the 120 kDa fragment was mediated by integrin α5β1. However, although a substrate-attached synthetic arginine-glycine-aspartic acid (RGD) peptide alone was able to promote the attachment and spreading of fibroblasts, it was inactive for hES cells, indicating that stem cells have different requirements in order to attach and spread on the central fibronectin RGD-cell-binding domain. This study provides further information on the characteristics of the cell-substrate interface required to control hES cell behaviour in clearly defined serum-free conditions, which are needed for the development of therapeutic applications of hES cells.
Deepak M Kalaskar; Joan E Downes; Patricia Murray; David H Edgar; Rachel L Williams
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-04-03
Journal Detail:
Title:  Journal of the Royal Society, Interface / the Royal Society     Volume:  10     ISSN:  1742-5662     ISO Abbreviation:  J R Soc Interface     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-04-04     Completed Date:  2013-09-16     Revised Date:  2014-06-08    
Medline Journal Info:
Nlm Unique ID:  101217269     Medline TA:  J R Soc Interface     Country:  England    
Other Details:
Languages:  eng     Pagination:  20130139     Citation Subset:  IM    
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MeSH Terms
Cell Adhesion
Cell Culture Techniques
Cellular Microenvironment*
Embryonic Stem Cells / cytology*
Extracellular Matrix / metabolism,  physiology
Fibronectins / chemistry*
Grant Support
BB/D014638/1//Biotechnology and Biological Sciences Research Council; G0300296//Medical Research Council
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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