Document Detail

Characterization of inositol phospholipids and identification of a mastoparan-induced polyphosphoinositide response in Tetrahymena pyriformis.
MedLine Citation:
PMID:  10907787     Owner:  NLM     Status:  MEDLINE    
The unicellular eukaryote Tetrahymena is a popular model for the study of lipid metabolism. Less attention, however, has been given to the inositol phospholipids of the cell, although it is known that this class of lipids plays an important role in eukaryotic cell signaling. Tetrahymena pyriformis phosphatidylinositol was isolated, purified, and characterized by proton nuclear magnetic resonance analysis and [2-(3)H]myoinositol labeling. Labeling was also used for polyphosphoinositide (phosphatidylinositol phosphate and phosphatidylinositol bisphosphate) identification. Tetrahymena inositol phospholipids were found to belong to the diacylglycerol group, although major Tetrahymena phospholipids, phosphatidylcholine and aminoethylphosphonoglycerides, have been found to be mainly alkylacylglyceroderivatives. Further characterization of Tetrahymena phosphatidylinositol by gas chromatographic analysis indicated that 80% of fatty acids were myristic acid and palmitic acid. This is also in contrast to the fatty acid profile of Tetrahymena phosphatidylcholine and phosphatidylethanolamine, with respect both to the fatty acid length and degree of unsaturation, and may indicate that specific diacylglycerol species are connected with the phosphatidylinositol metabolism in this cell. Treatment of [3H]inositol-labeled Tetrahymena cells with mastoparan, a G-protein-activating peptide, induced changes in the polyphosphoinositide levels, suggesting that inositol phospholipids may form in Tetrahymena a functional signaling system similar to that of higher eukaryotes. Addition of 10 microM mastoparan resulted in a rapid and transient increase in [3H]phosphatidylinositol phosphate followed by a decrease in [3H]phosphatidylinositol bisphosphate. Similar changes in lipids have been reported when phosphoinositide-phospholipase C pathway is activated in both animal and plant cells.
G Leondaritis; D Galanopoulou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  35     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-10-30     Completed Date:  2001-01-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  525-32     Citation Subset:  IM    
Department of Chemistry, University of Athens, Greece.
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MeSH Terms
Cells, Cultured
Chromatography, Thin Layer
Fatty Acids / chemistry
Lipids / isolation & purification
Magnetic Resonance Spectroscopy
Phosphatidylinositol Phosphates / chemistry*
Phosphatidylinositols / chemistry*
Signal Transduction
Tetrahymena / chemistry*
Time Factors
Wasp Venoms / pharmacology*
Reg. No./Substance:
0/Fatty Acids; 0/Lipids; 0/Peptides; 0/Phosphatidylinositol Phosphates; 0/Phosphatidylinositols; 0/Wasp Venoms; 72093-21-1/mastoparan

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