Document Detail


Characterization of the inner enamel epithelium in the enamel-free area based on the ability to secrete enamel protein demonstrated by in situ hybridization and immunohistochemistry.
MedLine Citation:
PMID:  9732123     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Both the expression of amelogenin mRNA and secretion of amelogenin were investigated in rat molars by in situ hybridization and immunohistochemistry. Probes were designed by multiple-labeling of oligonucleotide probes for in situ hybridization. Amelogenin mRNA first appeared in differentiating ameloblasts of the distal region and some inner enamel epithelial cells of enamel-free area (EFA cells) of the second cusp at postnatal day 0. At the same time, amelogenin protein was detected in the extracellular matrix between dentin and differentiating ameloblasts and in some EFA cells of the second cusp. At postnatal day 1-3, amelogenin was expressed in the secretory ameloblasts, and in the matrix beneath these cells. Both amelogenin mRNA and amelogenin were detected in the EFA cells and their extracellular matrix. After postnatal day 5, amelogenin mRNA and amelogenin were detected in the secretory ameoloblasts and extracellular matrix in the enamel-forming region, respectively. At this time, amelogenin mRNA was not detected in the EFA cells, but a small amount of amelogenin was found in the matrix beneath the EFA cells. These findings suggest that EFA cells differentiate into amelogenin-secreting cells, i.e. ameloblasts, but that the secretion lasts for only a short period at the early stage of tooth development.
Authors:
H Yamamoto; J Sasaki; J Matsuura; T Nomura; E F Sato; H Shimokawa; K Ishizeki; T Nawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta anatomica     Volume:  160     ISSN:  0001-5180     ISO Abbreviation:  Acta Anat (Basel)     Publication Date:  1997  
Date Detail:
Created Date:  1998-09-30     Completed Date:  1998-09-30     Revised Date:  2007-07-02    
Medline Journal Info:
Nlm Unique ID:  0370272     Medline TA:  Acta Anat (Basel)     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  232-8     Citation Subset:  IM    
Affiliation:
Department of Oral Anatomy, School of Dentistry, Iwate Medical University, Morioka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amelogenin
Animals
Animals, Newborn
Base Sequence
Dental Enamel Proteins / genetics,  metabolism*
Enamel Organ / cytology,  metabolism*
Epithelium / metabolism
Extracellular Matrix / metabolism
Female
Immunoenzyme Techniques
In Situ Hybridization
Molar / metabolism*
Molecular Sequence Data
Pregnancy
RNA, Messenger / metabolism
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Amelogenin; 0/Amelx protein, rat; 0/Dental Enamel Proteins; 0/RNA, Messenger

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