Document Detail

Characterization of the inflammatory infiltrate and cytokine expression in the skin of horses with recurrent urticaria.
MedLine Citation:
PMID:  23140316     Owner:  NLM     Status:  In-Data-Review    
Background -  Recurrent urticaria (RU) is a common skin disease of horses, but little is known about its pathogenesis. Hypothesis/Objective -  The aim of this study was to characterize the inflammatory cell infiltrate and cytokine expression pattern in the skin of horses with RU. Animals -  Biopsies of lesional and nonlesional skin of horses with RU (n = 8) and of skin from healthy control horses (n = 8) were evaluated. Methods -  The inflammatory cell infiltrate was analysed by routine histology. Immunohistochemistry was used to identify T cells (CD3), B cells (CD79), macrophages (MAC387) and mast cells (tryptase). Expression of T-helper 2 cytokines (interleukins IL-4, IL-5 and IL-13), a T-helper 1 cytokine (interferon-γ), IL-4 receptor α and thymic stromal lymphopoietin was assessed by quantitative RT-PCR. Results -  In subepidermal lesional skin of RU-affected horses, increased numbers of eosinophils (P ≤ 0.01), CD79-positive (P ≤ 0.01), MAC387-positive (P ≤ 0.01) and tryptase-positive cells (P ≤ 0.05) were found compared with healthy horses. Subepidermal lesional skin of RU-affected horses contained more eosinophils (P ≤ 0.05) and tryptase-positive cells (P ≤ 0.05) compared with nonlesional skin. There was no significant difference in infiltrating cells between nonlesional skin and skin of healthy horses. Expression of IL-4 (P ≤ 0.01), IL-13 (P ≤ 0.05), thymic stromal lymphopoietin (P ≤ 0.05) and IL-4 receptor α (P ≤ 0.05) was increased in lesional skin of RU-affected horses compared with control horses. Expression of IL-4 was higher (P ≤ 0.05) in lesional compared with nonlesional RU skin. Conclusions and clinical importance -  Analysis of cytokine expression and inflammatory infiltrate suggests that T-helper 2 cytokines, eosinophils, mast cells and presumptive macrophages play a role in the pathogenesis of equine RU.
Sandro Hinden; Jolanta Klukowska-Rötzler; Jozef Janda; Eliane I Marti; Vinzenz Gerber; Petra J Roosje
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Veterinary dermatology     Volume:  23     ISSN:  1365-3164     ISO Abbreviation:  Vet. Dermatol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9426187     Medline TA:  Vet Dermatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  503-e99     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Veterinary Dermatology © 2012 ESVD and ACVD.
Division of Clinical Dermatology and Department of Clinical Research-Veterinary Public Health, Vetsuisse Faculty, University of Bern, PO Box 8466, 3001 Bern, Switzerland Dermfocus, Vetsuisse Faculty, University of Bern, PO Box 8466, 3001 Bern, Switzerland Equine Clinic, Department of Clinical Veterinary Medicine, Vetsuisse Faculty, University of Bern, PO Box 8466, 3001 Bern, Switzerland.
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