| Characterization of the in vitro macrophage response and in vivo host response to poly(ethylene glycol)-based hydrogels. | |
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MedLine Citation:
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PMID: 19708075 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Photopolymerizable poly(ethylene glycol) (PEG)- based hydrogels have great potential as in vivo cell delivery vehicles for tissue engineering. However, their success in vivo will be dependent on the host response. The objectives for this study were to explore the in vivo host response and in vitro macrophage response to commonly used PEG-based hydrogels, PEG and PEG containing RGD. Acellular hydrogels were implanted subcutaneously into c57bl/6 mice and the foreign body response (FBR) was compared to medical grade silicone. Our findings demonstrated PEG-RGD hydrogels resulted in a FBR similar to silicone, while PEG-only hydrogels resulted in a robust inflammatory reaction characterized by a thick layer of macrophages at the material surface with evidence of gel degradation. In vitro, bone marrow-derived primary macrophages adhered well and similarly to PEG-based hydrogels, silicone, and tissue culture polystyrene when cultured for 4 days. Significantly higher gene expressions of the proinflammatory cytokines, TNF-alpha and Il-1beta, were found in macrophages seeded onto PEG compared to PEG-RGD and silicone at 1 and 2 days. PEG hydrogels were also shown to be susceptible to oxidative biodegradation. Our findings indicate that PEG-only hydrogels are proinflammatory while RGD attenuates this negative reaction leading to a moderate FBR. |
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Authors:
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Aaron D Lynn; Themis R Kyriakides; Stephanie J Bryant |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: 93 ISSN: 1552-4965 ISO Abbreviation: J Biomed Mater Res A Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-04-14 Completed Date: 2010-07-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: United States |
Other Details:
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Languages: eng Pagination: 941-53 Citation Subset: IM |
Affiliation:
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Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309-0424, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Marrow Cells / cytology Cell Count Cell Shape / drug effects Cytokines / genetics, metabolism Gene Expression Regulation / drug effects Hydrogels / pharmacology* Immunity / drug effects* Implants, Experimental Inflammation Mediators / metabolism Macrophages / cytology, drug effects*, immunology* Male Mice Mice, Inbred C57BL Oxidation-Reduction / drug effects Polyethylene Glycols / pharmacology* Silicones / pharmacology Solutions |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Hydrogels; 0/Inflammation Mediators; 0/Polyethylene Glycols; 0/Silicones; 0/Solutions |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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