Document Detail


Characterization and in vitro differentiation potential of a new human embryonic stem cell line, ReliCellhES1.
MedLine Citation:
PMID:  16533307     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human embryonic stem cells (hESCs) are an exceptionally useful tool for studies of human development and represent a potential source for transplantation therapies. At present, only a limited number of hESCs lines representing a very small sample of genetic diversity of the human populations are available. Here, we report the derivation and characterization of a new hESC line, ReliCellhES1. These cells, established from the inner cell mass (ICM) on mouse embryonic feeder (MEF) layer, satisfy the criteria that characterize pluripotent hESCs: The cell line expresses high levels of cell surface markers (such as SSEA-3, SSAEA-4, TRA-1-60 and TRA-1-81), transcription factor Oct-4, alkaline phosphatase (AP) and telomerase. The cell line retains normal karyotype in long-term culture and has a distinct identity as revealed by DNA fingerprinting by short tandem repeat (STR) analysis. Further, upon examination of the in vitro differentiation potential, ReliCellhES1 was found to be capable of giving rise to dopaminergic neurons, cardiomyocytes, pancreatic islets, and hepatocyte-like cells belonging to ectoderm, mesoderm, and endoderm lineages, respectively. To our knowledge, this is the first report of a well-characterized hES cell line from the Indian subcontinent.
Authors:
Arundhati Mandal; Shabari Tipnis; Rajarshi Pal; Geeta Ravindran; Bipasha Bose; Ameet Patki; Mahendra S Rao; Aparna Khanna
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  74     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-14     Completed Date:  2006-04-26     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  England    
Other Details:
Languages:  eng     Pagination:  81-90     Citation Subset:  IM    
Affiliation:
Embryonic stem cell group, Reliance Life Sciences Pvt. Ltd., Dhirubhai Ambani Life Sciences Center, R-282, TTC Industrial Area of MIDC Thane-Belapur Road, Rabale, Navi Mumbai 400 701, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / metabolism
Cell Differentiation
Cell Line*
Embryo, Mammalian / cytology*
Histocompatibility Testing
Humans
Karyotyping
Mice
Pluripotent Stem Cells / cytology*,  metabolism
Tandem Repeat Sequences
Telomerase / metabolism
Chemical
Reg. No./Substance:
0/Biological Markers; EC 2.7.7.49/Telomerase

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