Document Detail

Characterization and in vitro differentiation potential of a new human embryonic stem cell line, ReliCellhES1.
MedLine Citation:
PMID:  16533307     Owner:  NLM     Status:  MEDLINE    
Human embryonic stem cells (hESCs) are an exceptionally useful tool for studies of human development and represent a potential source for transplantation therapies. At present, only a limited number of hESCs lines representing a very small sample of genetic diversity of the human populations are available. Here, we report the derivation and characterization of a new hESC line, ReliCellhES1. These cells, established from the inner cell mass (ICM) on mouse embryonic feeder (MEF) layer, satisfy the criteria that characterize pluripotent hESCs: The cell line expresses high levels of cell surface markers (such as SSEA-3, SSAEA-4, TRA-1-60 and TRA-1-81), transcription factor Oct-4, alkaline phosphatase (AP) and telomerase. The cell line retains normal karyotype in long-term culture and has a distinct identity as revealed by DNA fingerprinting by short tandem repeat (STR) analysis. Further, upon examination of the in vitro differentiation potential, ReliCellhES1 was found to be capable of giving rise to dopaminergic neurons, cardiomyocytes, pancreatic islets, and hepatocyte-like cells belonging to ectoderm, mesoderm, and endoderm lineages, respectively. To our knowledge, this is the first report of a well-characterized hES cell line from the Indian subcontinent.
Arundhati Mandal; Shabari Tipnis; Rajarshi Pal; Geeta Ravindran; Bipasha Bose; Ameet Patki; Mahendra S Rao; Aparna Khanna
Related Documents :
3709597 - Heterogeneity of glycoprotein synthesis in human tumor cell lines.
9312077 - Development and age-associated differences in electron transport potential and conseque...
7679997 - Spontaneously arising immortal cell line of rat retinal pigmented epithelial cells.
19002917 - Characterization of rainbow trout cell lines using microsatellite dna profiling.
20445347 - Anti-melanoma activity of the 9.2.27pe immunotoxin in dacarbazine resistant cells.
21528267 - Overexpression of activin a inhibits growth, induces apoptosis, and suppresses tumorige...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  74     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-14     Completed Date:  2006-04-26     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  England    
Other Details:
Languages:  eng     Pagination:  81-90     Citation Subset:  IM    
Embryonic stem cell group, Reliance Life Sciences Pvt. Ltd., Dhirubhai Ambani Life Sciences Center, R-282, TTC Industrial Area of MIDC Thane-Belapur Road, Rabale, Navi Mumbai 400 701, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Biological Markers / metabolism
Cell Differentiation
Cell Line*
Embryo, Mammalian / cytology*
Histocompatibility Testing
Pluripotent Stem Cells / cytology*,  metabolism
Tandem Repeat Sequences
Telomerase / metabolism
Reg. No./Substance:
0/Biological Markers; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The Muscleblind family of proteins: an emerging class of regulators of developmentally programmed al...
Next Document:  Cells differentiated from mouse embryonic stem cells via embryoid bodies express renal marker molecu...