Document Detail


Characterization in primary monolayer culture of separated cell types from rabbit endometrium.
MedLine Citation:
PMID:  6337259     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Epithelial cells and stromal cells of the rabbit endometrium were separated by successive enzymic digestion of the uterine mucosa. Isolated cell types were obtained in high yield, with good viability, and were maintained in monolayer cultures for up to 2 weeks. Epithelial cells in monolayers appeared as polygonal cells, displayed contact inhibition, and showed the presence of microvilli on the cell surface, with many desmosomes. Stromal cells grew rapidly to confluence, displayed overgrowth, and had a fibroblastic appearance with an absence of junctional complexes between cells. Indirect immunofluorescence showed uteroglobin on the surface of epithelial but not of stromal cells, and only epithelial cells secreted uteroglobin into the medium. These results confirm the identity of the cells and provide biochemical evidence for the epithelial cellular origin of uteroglobin. The method allows the culture of separate endometrial cell types, which retain their morphology and differentiated function in vitro.
Authors:
A P Ricketts; M Hagensee; D W Bullock
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  67     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1983 Jan 
Date Detail:
Created Date:  1983-03-11     Completed Date:  1983-03-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  151-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Separation / methods
Cells, Cultured
Endometrium / ultrastructure*
Epithelium / secretion,  ultrastructure
Female
Fluorescent Antibody Technique
Microscopy, Electron
Pregnancy
Rabbits
Uteroglobin / biosynthesis,  secretion
Grant Support
ID/Acronym/Agency:
HD 13663/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
9060-09-7/Uteroglobin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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