| Characterization of immune responses induced by combined Clade-A HIV-1 recombinant Adenovectors in mice. | |
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MedLine Citation:
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PMID: 20876987 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background: Numerous evidences indicate that in some HIV-1 positive patients, the humoral and cellular immune responses are induced against HIV-1 proteins and this is inversely related to the progress of infection. Objective: The aim of this study was the evaluation of the Adenovectors containing HIV genes in induction of immune responses in mice. Methods: The HIV-1 genes including gag p24, rev, nef and exon-1 of tat were amplified from HIV-1 RNA (clade-A). The cDNA of each gene was cloned into a transfer vector. The transfer vector was then co-transformed into E. coli strain BJ5183 together with pAdenovector ∆E1/E3. The recombinant adenoviral construct was transfected into QBI-293A cells. Recombinant viruses were purified and titrated on 293 cell plates. Expression of transgenes was evaluated using western blotting. Then 10E12 viral particles were injected into 15 groups of 5 mice and all patterns of combination of these 4 HIV-1 genes were evaluated. After 2 weeks, humoral and cellular immune responses were evaluated using ELISA, cell proliferation and ELISpot (IL-2, IL-4 and IFN-γ) assays, consecutively. Results: It was demonstrated that each gene was expressed. The response targets were mostly toward Th1, though several Th2 responses were also observed. Single injection in our study induced a good cellular response but the humoral responses were not as strong as the cellular ones. Conclusion: Considering and comparing all results and evaluating the various possible interactions revealed that simultaneous injection of tat and gag has enhanced the humoral and cellular responses. |
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Authors:
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Saeed Bayanolhagh; Mahtab Alinezhad; Koorosh Kamali; Maryam Foroughi; Hamid Reza Khorram Khorshid; Minoo Mohraz; Fereidoun Mahboudi; Ali Akbar Pourfathollah |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Iranian journal of immunology : IJI Volume: 7 ISSN: 1735-1383 ISO Abbreviation: Iran J Immunol Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101282932 Medline TA: Iran J Immunol Country: Iran |
Other Details:
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Languages: eng Pagination: 162-76 Citation Subset: IM |
Affiliation:
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Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran, e-mail: pourfa@modares.ac.ir. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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