Document Detail

Characterization and identification of exflagellation-inducing factor in the salivary gland of Anopheles stephensi (Diptera: Culicidae).
MedLine Citation:
PMID:  11573943     Owner:  NLM     Status:  MEDLINE    
Gamete activation factor (GAF) induces exflagellation of Plasmodium microgametes. We found GAF in the salivary glands of female mosquitoes, Anopheles stephensi. The exflagellation was induced in a concentration-dependent manner in the supernatant of salivary gland's crude homogenate. The exflagellation-inducing activity in the salivary gland was higher than that in the midgut and the head. GAF in the salivary glands was found to be heat stable and low molecular weight (<3000 molecular weight). Analysis of the supernatant by capillary electrophoresis and UV absorbance profile showed that the salivary glands contained xanthurenic acid, which was previously identified as GAF in the head of A. stephensi. The exflagellation-inducing activity in the salivary gland declined immediately after a blood meal, implying that GAF was in the saliva, and was delivered into the midgut together with the blood and induced exflagellation in the midgut.
M Hirai; J Wang; S Yoshida; A Ishii; H Matsuoka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  287     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-09-27     Completed Date:  2001-12-04     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  859-64     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Department of Medical Zoology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi, Tochigi 329-0498, Japan.
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MeSH Terms
Anopheles / chemistry,  parasitology,  physiology*
Flagella / metabolism*
Hot Temperature
Hydrogen-Ion Concentration
Molecular Weight
Plasmodium berghei / physiology*
Salivary Glands / chemistry,  metabolism
Spectrophotometry, Ultraviolet
Tissue Extracts / chemistry,  pharmacology
Xanthurenates / chemistry,  metabolism*
Reg. No./Substance:
0/Tissue Extracts; 0/Xanthurenates; 59-00-7/xanthurenic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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