Document Detail

Characterization of hydrogen peroxide production by Duox in bronchial epithelial cells exposed to Pseudomonas aeruginosa.
MedLine Citation:
PMID:  20085766     Owner:  NLM     Status:  MEDLINE    
Hydrogen peroxide production by the NADPH oxidase Duox1 occurs during activation of respiratory epithelial cells stimulated by purified bacterial ligands, such as lipopolysaccharide. Here, we characterize Duox activation using intact bacterial cells of several airway pathogens. We found that only Pseudomonas aeruginosa, not Burkholderia cepacia or Staphylococcus aureus, triggers H2O2 production in bronchial epithelial cells in a calcium-dependent but predominantly ATP-independent manner. Moreover, by comparing mutant Pseudomonas strains, we identify several virulence factors that participate in Duox activation, including the type-three secretion system. These data provide insight on Duox activation by mechanisms unique to P. aeruginosa.
Balázs Rada; Thomas L Leto
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2010-01-19
Journal Detail:
Title:  FEBS letters     Volume:  584     ISSN:  1873-3468     ISO Abbreviation:  FEBS Lett.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-23     Completed Date:  2010-03-16     Revised Date:  2011-07-26    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  917-22     Citation Subset:  IM    
National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda 20852, USA.
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MeSH Terms
Adenosine Triphosphate / metabolism
Bacterial Proteins / genetics,  physiology
Blotting, Western
Burkholderia cepacia / physiology
Calcium / metabolism
Cell Line
Epithelial Cells / metabolism*,  microbiology*
Hydrogen Peroxide / metabolism*
NADPH Oxidase / genetics,  metabolism*
Pseudomonas aeruginosa / metabolism,  physiology*
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
Staphylococcus aureus / physiology
Virulence Factors / genetics,  physiology
Grant Support
Reg. No./Substance:
0/Bacterial Proteins; 0/Virulence Factors; 56-65-5/Adenosine Triphosphate; 7440-70-2/Calcium; 7722-84-1/Hydrogen Peroxide; EC protein, human; EC Oxidase

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