Document Detail

Characterization of human papillomavirus type 57b: transforming activity and comparative sequence analysis as probes for biological determinants associated with high-risk oncogenic viruses.
MedLine Citation:
PMID:  8879133     Owner:  NLM     Status:  MEDLINE    
The association of human papillomavirus type 57 (HPV-57) with premalignant and malignant tumors of the nasal cavity was previously reported (Wu et al., Lancet 341, 522, 1993). We determined the complete nucleotide sequence of HPV-57b (GenBank 37537), which was molecularly cloned from a benign fungiform papilloma, and compared it with other HPV types and HPV-57a, which was cloned from an inverted papilloma of the maxillary sinus by de Villiers et al. (Virology 171, 248. 1989). Comparative and phylogenetic analysis of amino acid sequences of the HPV-57b oncogenes E5, E6, and E7 were performed with HPV-6, 11, 16, and 18. Phylogenetic trees using the Jotun-Hein algorithm indicated a closer relationship of HPV-57b E5 and E7 with corresponding genes of HPV-18. Signature pattern analysis of these two oncogenes was also in agreement with a closer relatedness to HPV-16 and 18 oncogenes, which are associated with a high risk for malignant progression. Compared with 7861 bp of HPV-57a, HPV-57b had 7868 bp as well as differences in the restriction enzyme sites and the open reading frames, including at least five additional ones. To investigate the oncogenic potential of HPV-57b, NIH 3T3 and REF52 cells were cotransfected with two plasmids: pKP54. HPV-57b, which contains the HPV-57b genome, and pMT.neo.1, which confers resistance to G418. After selection in culture medium containing G418, 58% of the G418r NIH 3T3 colonies and 47% of the G418r REF52 colonies exhibited morphological transformation. These results indicate that the transcriptional regulatory elements and the oncoproteins of HPV-57b are active in vitro to induce cellular transformation, as are other high-risk HPV types.
J M Trujillo; T C Wu; P Mounts
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Virus genes     Volume:  12     ISSN:  0920-8569     ISO Abbreviation:  Virus Genes     Publication Date:  1996  
Date Detail:
Created Date:  1997-01-14     Completed Date:  1997-01-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8803967     Medline TA:  Virus Genes     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  165-78     Citation Subset:  IM    
Department of Molecular Microbiology and Immunology, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205, USA.
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MeSH Terms
3T3 Cells
Base Sequence
DNA, Viral
Molecular Sequence Data
Papillomaviridae / genetics*
Transformation, Genetic
Reg. No./Substance:
0/DNA, Viral

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