| Characterization of human melanoma cell lines according to their migratory properties in vitro. | |
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MedLine Citation:
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PMID: 14692828 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The migratory responses of four human melanoma cell lines (A-2058, DEMEL, HTB-63, and HTB-72), using chemotaxis (CTX) and haptotaxis (HPTX) assays, were studied. The attractants were three extracellular matrix components (EMCs), fibronectin, laminin, and collagen type IV. The conditioned media (CM) of each cell line were used to study autocrine and paracrine responses. A screening and sensitive CTX assay was performed, using pertussis toxin (PTX)- treated A-2058 as responder cells; the other melanoma cells and normal cells were used as secretory cells. Autotaxin (ATX), a purified autocrine motility factor, was also used as a chemoattractant. Reverse transcriptase-polymerase chain reaction was used to detect the expression of ATX by all cell lines. The secretion of ATX was determined by Western blot. The invasive capacity of the cell lines was evaluated using Matrigel and ATX as attractant. Chemotaxis responses to EMCs varied. Except for the A-2058 cells, HPTX migration was low. Autocrine and paracrine responses also varied. The migration of PTX-treated A-2058 cells to ATX and to their own CM was abolished. All the melanoma cells expressed ATX, and except for the HTB-72 and normal cells, all secreted ATX. Matrigel was invaded by all the melanoma cell lines except the HTB-72 and normal cells. The migratory properties of human melanoma cells in vitro suggest that they could correlate to their metastatic potential in vivo. |
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Authors:
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Luis G Quiñones; Ivette Garcia-Castro |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: In vitro cellular & developmental biology. Animal Volume: 40 ISSN: 1071-2690 ISO Abbreviation: In Vitro Cell. Dev. Biol. Anim. Publication Date: 2004 Jan-Feb |
Date Detail:
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Created Date: 2004-06-07 Completed Date: 2005-02-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9418515 Medline TA: In Vitro Cell Dev Biol Anim Country: United States |
Other Details:
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Languages: eng Pagination: 35-42 Citation Subset: IM |
Affiliation:
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Biology Department, University of Puerto Rico, Río Piedras Campus, P.O. Box 22283, San Juan, PR 00931-2283. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autocrine Communication Biological Assay Cell Line, Tumor* Cell Movement / physiology* Chemotactic Factors / metabolism Collagen / metabolism Collagen Type IV / metabolism Culture Media, Conditioned Drug Combinations Extracellular Matrix / chemistry Female Fibronectins / metabolism Humans Laminin / metabolism Male Melanoma* / metabolism, pathology Paracrine Communication Pertussis Toxin / metabolism Proteoglycans / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Chemotactic Factors; 0/Collagen Type IV; 0/Culture Media, Conditioned; 0/Drug Combinations; 0/Fibronectins; 0/Laminin; 0/Proteoglycans; 119978-18-6/matrigel; 9007-34-5/Collagen; EC 2.4.2.31/Pertussis Toxin |
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