Document Detail


Characterization of human maligant melanoma cell lines. I. Morphology and growth characteristics in culture.
MedLine Citation:
PMID:  1127734     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Seven human malignant melanoma lines were maintained in vitro for various periods of time. One line, established in this laboratory from a metastatic solid tumor by repeated treatment of the primary outgrowth with 0.02 percent EDTA, allowed a continuous culture of melanoma cells free of fibroblasts. By light microscopy, cells in each line could be classified into one of three morphologic types: elongated dendritic, cuboidal, or triangular dendritic. Four of the seven lines exhibited various degrees of pigmentation. The growth pattern was determined by plating efficiency and saturation density for each line. Cytogenetic analysis with the fluorescent banding technique revealed only human chromosomes with gross aneuploidy. Major marker chromosomes specific for each line were identified. None of the parameters studied showed any correlation or interdependence with one another, except for an association of elongated dendritic morphology with poor plating efficiency and low saturation density.
Authors:
S K Liao; P B Dent; P B McCulloch
Related Documents :
16486334 - Constraints on changes in fundamental constants from a cosmologically distant oh absorb...
8139534 - A gene involved in control of human cellular senescence on human chromosome 1q.
10531554 - Production and propagation of fully inbred clonal lines in the nile tilapia (oreochromi...
1127734 - Characterization of human maligant melanoma cell lines. i. morphology and growth charac...
21180244 - Chronic myeloid leukemia--from the national to the european registry--limited experienc...
1137344 - Chromosome assignment of some human enzyme loci: mitochondrial malate dehydrogenase to ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of the National Cancer Institute     Volume:  54     ISSN:  0027-8874     ISO Abbreviation:  J. Natl. Cancer Inst.     Publication Date:  1975 May 
Date Detail:
Created Date:  1975-08-08     Completed Date:  1975-08-08     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7503089     Medline TA:  J Natl Cancer Inst     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1037-44     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aneuploidy
Cell Division
Cell Line*
Chromosomes / ultrastructure
Humans
Melanoma / genetics,  pathology*
Pigmentation
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Social class differences among patients with large-bowel cancer in Cali, Colombia.
Next Document:  Circulating interferon in man after administration of exogenous human leukocyte interferon.