Document Detail


Characterization of human PA2.26 antigen (T1alpha-2, podoplanin), a small membrane mucin induced in oral squamous cell carcinomas.
MedLine Citation:
PMID:  15515019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report the full cDNA sequence encoding the human homologue of murine PA2.26 (T1alpha-2, podoplanin), a small mucin-type transmembrane glycoprotein originally identified as a cell-surface antigen induced in keratinocytes during mouse skin carcinogenesis. The human PA2.26 gene is expressed as 2 transcripts of 0.9 and 2.7 kb in several normal tissues, such as the placenta, skeletal muscle, heart and lung. Using a specific polyclonal antibody raised against a synthetic peptide of the protein ectodomain, PA2.26 was immunohistochemically detected in about 25% (15/61) of human early oral squamous cell carcinomas. PA2.26 distribution in the tumours was heterogeneous and often restricted to the invasive front. Double immunofluorescence and confocal microscopy analysis showed that PA2.26 colocalized with the membrane cytoskeleton linker ezrin at the surface of tumour cells and that its presence in vivo was associated with downregulation of membrane E-cadherin protein expression. Ectopic expression of human PA2.26 in HeLa carcinoma cells and immortalized HaCaT keratinocytes promoted a redistribution of ezrin to the cell edges, the formation of cell-surface protrusions and reduced Ca(2+)-dependent cell-cell adhesiveness. These results point to PA2.26 as a novel biomarker for oral squamous cell carcinomas that might be involved in migration/invasion.
Authors:
Ester Martín-Villar; Francisco G Scholl; Carlos Gamallo; Maria M Yurrita; Mario Muñoz-Guerra; Jesús Cruces; Miguel Quintanilla
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  113     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2004-12-27     Completed Date:  2005-03-07     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  899-910     Citation Subset:  IM    
Copyright Information:
(c) 2004 Wiley-Liss, Inc.
Affiliation:
Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas (CSIC)-Universidad Autónoma de Madrid (UAM), Madrid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antigens, Surface / genetics*
Base Sequence
Carcinoma, Squamous Cell / genetics*,  pathology
Female
Humans
Membrane Glycoproteins / genetics*
Mice
Molecular Sequence Data
Mouth Neoplasms / genetics*,  pathology
Neoplasm Invasiveness
Neoplasm Staging
Organ Specificity
Pregnancy
Protein Biosynthesis
RNA, Messenger / genetics
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Antigens, Surface; 0/Gp38 protein, mouse; 0/Membrane Glycoproteins; 0/RNA, Messenger

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