| Characterization and hormonal modulation of anticoagulant heparan sulfate proteoglycans synthesized by rat ovarian granulosa cells. | |
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MedLine Citation:
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PMID: 8703018 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Anticoagulant heparan sulfate proteoglycans endow the vascular endothelium with antithrombotic properties, but their role outside the vascular bed is unknown. Granulosa cells form an avascular compartment in the ovarian follicle, in which a heparin-like activity has been described. At ovulation extravascular coagulation occurs around ovulatory follicles, and after expulsion of the oocyte, a fibrin clot forms in the antral cavity. Granulosa cells synthesize two major heparan sulfate proteoglycans, whose anticoagulant nature has not been investigated. The purpose of this study was to characterize anticoagulant heparan sulfate proteoglycans synthesized by rat ovarian granulosa cells. Affinity purified 35S-labeled anticoagulant heparan sulfate glycosaminoglycans represent 6.5% of the total heparan sulfate synthesized, and they contain 13% 3-O-sulfated disaccharides that are markers of the antithrombin-binding site of heparin. The biological activity of granulosa cell heparan sulfate proteoglycans was demonstrated by their ability to bind antithrombin and to accelerate the formation of thrombin-antithrombin complexes. The impact of hormonal stimulation on granulosa cell anticoagulant heparan sulfate proteoglycans was studied using 125I-antithrombin binding assays. Follicle-stimulating hormone induced a redistribution of anticoagulant heparan sulfate proteoglycans from the granulosa cell layer to the culture medium, indicating that their distribution could be modulated according to the stage of follicular development. These results suggest that anticoagulant heparan sulfate might be critically located in the follicle to maintain fluidity around the oocyte until its expulsion at ovulation. |
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Authors:
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G Hosseini; J Liu; A I de Agostini |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 271 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1996 Sep |
Date Detail:
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Created Date: 1996-10-10 Completed Date: 1996-10-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 22090-9 Citation Subset: IM |
Affiliation:
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Department of Gynaecology and Obstetrics, Geneva University Hospital, CH 1211 Geneva 14, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anticoagulants / metabolism* Antithrombin III / metabolism Cell Adhesion Cells, Cultured Chromatography, Affinity Disaccharides / analysis Electrophoresis, Polyacrylamide Gel Endothelium / metabolism Estradiol / pharmacology Female Follicle Stimulating Hormone / pharmacology Granulosa Cells / drug effects, metabolism* Heparan Sulfate Proteoglycans Heparitin Sulfate / biosynthesis*, isolation & purification Molecular Weight Proteoglycans / biosynthesis*, isolation & purification Rats Rats, Sprague-Dawley Sulfates / metabolism Thrombin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anticoagulants; 0/Disaccharides; 0/Heparan Sulfate Proteoglycans; 0/Proteoglycans; 0/Sulfates; 50-28-2/Estradiol; 9000-94-6/Antithrombin III; 9002-68-0/Follicle Stimulating Hormone; 9050-30-0/Heparitin Sulfate; EC 3.4.21.5/Thrombin |
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