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Characterization of homologous DNA recombination activity in normal and immortal mammalian cells.
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MedLine Citation:
PMID:  8918816     Owner:  NLM     Status:  MEDLINE    
Homologous DNA recombination levels were measured in normal and spontaneously immortalized murine and human fibroblasts, and in a number of primate and murine established fibroblast cell lines. Immortal cell lines and tumor-derived clones homologously recombined extrachromosomal plasmid substrates at frequencies approximately 100-fold higher than did normal cells. To further explore the mechanism responsible for this phenotype, homologous recombination frequency was measured using nuclear extracts derived from normal and immortalized murine and human fibroblasts. Extracts prepared from immortal cells catalyzed high levels of homologous recombination, whereas very little recombination activity was detected in extracts prepared from normal fibroblasts. Similarly, only extracts derived from immortal cells contained strand-transferase activity as measured by the recently described pairing-on-membrane assay. Mixing experiments indicated that a recombination enhancing factor or factors present in immortal cells, rather than a recombination inhibitor in normal cells, was responsible for the enhanced homologous recombination activity observed using extracts derived from the former.
B Thyagarajan; M McCormick-Graham; D P Romero; C Campbell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nucleic acids research     Volume:  24     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1996-12-18     Completed Date:  1996-12-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  4084-91     Citation Subset:  IM    
Department of Pharmacology, University of Minnesota Medical School, Minneapolis 55455, USA.
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MeSH Terms
DNA / metabolism*
Electrophoresis, Agar Gel
Nuclear Proteins / metabolism
Nucleotidyltransferases / metabolism
Plasmids / metabolism
Recombination, Genetic / genetics*
Transfection / genetics
Tumor Cells, Cultured
Reg. No./Substance:
0/Nuclear Proteins; 9007-49-2/DNA; EC 2.7.7.-/Nucleotidyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 15 Month: 10 Year: 1996
Volume: 24 Issue: 20
First Page: 4084 Last Page: 4091
ID: 146187
PubMed Id: 8918816
Publisher Item Identifier: 6g0229

Characterization of homologous DNA recombination activity in normal and immortal mammalian cells.
B Thyagarajan
M McCormick-Graham
D P Romero
C Campbell
Department of Pharmacology, University of Minnesota Medical School, Minneapolis 55455, USA.

Article Categories:
  • Research Article

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