Document Detail


Characterization of high affinity neurotensin receptor NTR1 in HL-60 cells and its down regulation during granulocytic differentiation.
MedLine Citation:
PMID:  10193787     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. We investigated responses to neurotensin in human promyelocytic leukaemia HL-60 cells. 2. Neurotensin increased the cytosolic calcium concentration ([Ca2+]i) in a concentration-dependent manner and also produced inositol 1,4,5-trisphosphate (InsP3). 3. Among the tested neurotensin analogues, neurotensin 8-13, neuromedin-N, and xenopsin also increased [Ca2+]i, whereas neurotensin 1-11 and neurotensin 1-8 did not elicit detectable responses. 4. SR48692, an antagonist of NTR1 neurotensin receptors, blocked the neurotensin-induced [Ca2+]i increase, whereas levocabastine, which is known as an NTR2 neurotensin receptor antagonist, did not attenuate the neurotensin-evoked effect. 5. The expression of NTR1 neurotensin receptors was confirmed by Northern blot analysis and reverse transcriptase-polymerase chain reaction (RT-PCR). 6. During 1.25% dimethylsulfoxide (DMSO)-triggered granulocytic differentiation of HL-60 cells, the neurotensin-induced [Ca2+]i rise became gradually smaller and completely disappeared 4 days after treatment with DMSO. The mRNA level for neurotensin receptors was also decreased after differentiation. 7. The results show that HL-60 cells express NTR1 neurotensin receptors and suggest that granulocytic differentiation involves transcriptional regulation of the receptors resulting in down-regulation of the neurotensin-induced signalling.
Authors:
S Y Choi; H D Chae; T J Park; H Ha; K T Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of pharmacology     Volume:  126     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-05-25     Completed Date:  1999-05-25     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1050-6     Citation Subset:  IM    
Affiliation:
Department of Life Science, Pohang University of Science and Technology, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Calcium / metabolism
Cell Differentiation
Cyclic AMP / biosynthesis
Down-Regulation
Granulocytes / chemistry*,  cytology
HL-60 Cells / chemistry*
Humans
Neurotensin / pharmacology
Pyrazoles / pharmacology
Quinolines / pharmacology
Receptors, Neurotensin / analysis*
Chemical
Reg. No./Substance:
0/Pyrazoles; 0/Quinolines; 0/Receptors, Neurotensin; 146362-70-1/SR 48692; 39379-15-2/Neurotensin; 60-92-4/Cyclic AMP; 7440-70-2/Calcium
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