Document Detail


Characterization of the heterochromatic chromosome regions in sheep.
MedLine Citation:
PMID:  9546206     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to elucidate the structural chromosome organization of the heterochromatic regions in sheep, we have used C-banding, silver-staining, sequential CDD technique and restriction endonuclease banding. By these banding techniques we obtained four fractions of repetitive DNA, the autosomal fractions A and B, the C fraction in the X chromosome, and the D fraction in the Y chromosome. Silver staining revealed active nucleolus organizer regions (NOR's) on the telomeric GC-rich areas of chromosomes 1, 2, 3, 4, and 25 which were digested with HaeIII restriction endonuclease.
Authors:
J L Fernández-García; M Martínez-Trancón; A Rabasco; J A Padilla
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Genes & genetic systems     Volume:  73     ISSN:  1341-7568     ISO Abbreviation:  Genes Genet. Syst.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-05-04     Completed Date:  1998-05-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9607822     Medline TA:  Genes Genet Syst     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  45-50     Citation Subset:  IM    
Affiliation:
Departamento de Zootecnia, Genética y Mejora Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosome Banding*
Chromosomes / chemistry*
DNA Restriction Enzymes
Female
Heterochromatin*
Karyotyping
Male
Nucleolus Organizer Region
Sheep / genetics*
Silver Staining
Staining and Labeling
Telomere
X Chromosome / chemistry
Y Chromosome / chemistry
Chemical
Reg. No./Substance:
0/Heterochromatin; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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