Document Detail

Characterization of gonadotropin-releasing hormone binding to pituitary receptors in the gilthead seabream (Sparus aurata).
MedLine Citation:
PMID:  1337273     Owner:  NLM     Status:  MEDLINE    
Receptors for GnRH in the pituitary of Sparus aurata were characterized using iodinated [D-Ala6-Pro9-NEt]-LHRH (GnRHa). Equilibrium binding of the ligand to the receptor was achieved after 1 h at 4 degrees C. Binding of the radioligand was a function of tissue concentration, with a linear correlation over the range of one-sixteenth to three-fourths pituitary per tube. Displacement experiments with salmon GnRH (sGnRH), GnRHa, as well as unrelated peptides demonstrated the specificity of the receptors. Binding was found to be saturable at ligand concentrations of 4 x 10(-9) M. Scatchard analysis of the saturation data suggested the presence of a single class of high-affinity sites (Ka = 0.567 +/- 0.136 x 10(9) M-1, Bmax = 1091 +/- 207 fmol/mg protein).
G Pagelson; Y Zohar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  47     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-03-10     Completed Date:  1993-03-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1004-8     Citation Subset:  IM    
National Center for Mariculture, Israel Oceanographic and Limnological Research Institute, Eilat.
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MeSH Terms
Fishes / metabolism*
Gonadotropin-Releasing Hormone / metabolism*
Least-Squares Analysis
Pituitary Gland / metabolism*
Receptors, LHRH / metabolism*
Substrate Specificity
Time Factors
Reg. No./Substance:
0/Receptors, LHRH; 33515-09-2/Gonadotropin-Releasing Hormone

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