Document Detail


Characterization of gonadotropin-releasing hormone binding to pituitary receptors in the gilthead seabream (Sparus aurata).
MedLine Citation:
PMID:  1337273     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Receptors for GnRH in the pituitary of Sparus aurata were characterized using iodinated [D-Ala6-Pro9-NEt]-LHRH (GnRHa). Equilibrium binding of the ligand to the receptor was achieved after 1 h at 4 degrees C. Binding of the radioligand was a function of tissue concentration, with a linear correlation over the range of one-sixteenth to three-fourths pituitary per tube. Displacement experiments with salmon GnRH (sGnRH), GnRHa, as well as unrelated peptides demonstrated the specificity of the receptors. Binding was found to be saturable at ligand concentrations of 4 x 10(-9) M. Scatchard analysis of the saturation data suggested the presence of a single class of high-affinity sites (Ka = 0.567 +/- 0.136 x 10(9) M-1, Bmax = 1091 +/- 207 fmol/mg protein).
Authors:
G Pagelson; Y Zohar
Related Documents :
8469773 - [125i]cgp 42112 binding reveals differences between rat brain and adrenal at2 receptor ...
1467963 - Angiotensin ii binding sites in the hamster brain: localization and subtype distribution.
11287563 - The e8 domain confers a novel long-distance transcriptional repression activity on the ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  47     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-03-10     Completed Date:  1993-03-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1004-8     Citation Subset:  IM    
Affiliation:
National Center for Mariculture, Israel Oceanographic and Limnological Research Institute, Eilat.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Female
Fishes / metabolism*
Gonadotropin-Releasing Hormone / metabolism*
Least-Squares Analysis
Male
Pituitary Gland / metabolism*
Receptors, LHRH / metabolism*
Substrate Specificity
Time Factors
Chemical
Reg. No./Substance:
0/Receptors, LHRH; 33515-09-2/Gonadotropin-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Preparation of a stable liquid material for calibration and quality control for lysosomal enzymes in...
Next Document:  Stimulation of phospholipase A2 by xanthine oxidase in the rat corpus luteum.