Document Detail


Characterization of glycoside hydrolase family 6 enzymes from Coprinopsis cinerea.
MedLine Citation:
PMID:  19502758     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recombinants, Cel6 from Coprinopsis cinerea (CcCel6)A, -B, and -C, were expressed by Escherichia coli. These enzymes produced cellobiose from phosphoric acid-swollen cellulose. When Avicel was used as the substrate, CcCel6A showed higher activity toward the substrate than CcCel6B or -C. In reaction with carboxymethylcellulose, CcCel6B and -C hydrolyzed the substrate, whereas CcCel6A failed to react.
Authors:
Yuan Liu; Kiyohiko Igarashi; Satoshi Kaneko; Takashi Tonozuka; Masahiro Samejima; Kiyoharu Fukuda; Makoto Yoshida
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  73     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-24     Completed Date:  2009-10-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1432-4     Citation Subset:  IM    
Affiliation:
Department of Environmental and Natural Resource Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Basidiomycota / enzymology*
Chromatography, Thin Layer
DNA Primers
Electrophoresis, Polyacrylamide Gel
Glycoside Hydrolases / metabolism*
Substrate Specificity
Chemical
Reg. No./Substance:
0/DNA Primers; EC 3.2.1.-/Glycoside Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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