Document Detail

Characterization of glycosaminoglycans produced by primary astrocytes in vitro.
MedLine Citation:
PMID:  1832659     Owner:  NLM     Status:  MEDLINE    
Quantitative biosynthetic studies with cultures highly enriched for glial fibrillary acidic protein (GFAP+) cells of neonatal mammalian brain demonstrated production of four proteoglycans: hyaluronate (HA), heparan sulphate (HS), chondroitin sulphate (CS), and dermatan sulphate (DS). The glycosaminoglycans were present in cell conditioned medium and in the cellular compartment. There were qualitative differences in the subcellular disposition of the various proteoglycans. The ratio of HS to CS/DS in cell extracts was 1:1, while in medium this ratio was 1:6. All of the glycosaminoglycans were associated with core proteins that were integral to the cell membrane and associated with the cell surface by non-covalent interactions involving glycosaminoglycans. Less than 20% of the HS was non-covalently associated with the astrocyte cell surface reflecting in part the proportionately smaller amounts of this proteoglycan released to astrocyte conditioned medium. HS released to medium was undersulphated relative to that associated with cells. The astrocyte can contribute proteoglycans to the extracellular milieu and displays cell surface proteoglycans that have the potential to provide appropriate substrates for neuron adhesion, process extension, and other cell-cell interactions.
P C Johnson-Green; K E Dow; R J Riopelle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Glia     Volume:  4     ISSN:  0894-1491     ISO Abbreviation:  Glia     Publication Date:  1991  
Date Detail:
Created Date:  1991-10-18     Completed Date:  1991-10-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8806785     Medline TA:  Glia     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  314-21     Citation Subset:  IM    
Department of Medicine, Queen's University, Kingston, Ontario, Canada.
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MeSH Terms
Astrocytes / metabolism*
Cells, Cultured
Chondroitin Sulfates / biosynthesis
Dermatan Sulfate / biosynthesis
Glial Fibrillary Acidic Protein / metabolism
Glycosaminoglycans / biosynthesis*
Heparitin Sulfate / biosynthesis
Hyaluronic Acid / biosynthesis
Proteoglycans / biosynthesis
Grant Support
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein; 0/Glycosaminoglycans; 0/Proteoglycans; 24967-94-0/Dermatan Sulfate; 9004-61-9/Hyaluronic Acid; 9007-28-7/Chondroitin Sulfates; 9050-30-0/Heparitin Sulfate

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