| Characterization of glutamate decarboxylase from a high gamma-aminobutyric acid (GABA)-producer, Lactobacillus paracasei. | |
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MedLine Citation:
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PMID: 18256502 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Gamma-aminobutyric acid (GABA) has several physiological functions in humans. We have reported that Lactobacillus paracasei NFRI 7415 produces high levels of GABA. To gain insight into the higher GABA-producing ability of this strain, we analyzed glutamate decarboxylase (GAD), which catalyzes the decarboxylation of L-glutamate to GABA. The molecular weight of the purified GAD was estimated to be 57 kDa by SDS-PAGE and 110 kDa by gel filtration, suggesting that GAD forms the dimer under native conditions. GAD activity was optimal at pH 5.0 at 50 degrees C. The Km value for the catalysis of glutamate was 5.0 mM, and the maximum rate of catalysis was 7.5 micromol min(-1) mg(-1). The N-terminal amino acid sequence of GAD was determined, and the gene encoding GAD from genomic DNA was cloned. The findings suggest that the ability of Lb. paracasei to produce high levels of GABA results from two characteristics of GAD, viz., a low Km value and activity at low pH. |
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Authors:
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Noriko Komatsuzaki; Toshihide Nakamura; Toshinori Kimura; Jun Shima |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-02-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 72 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-02-25 Completed Date: 2008-08-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 278-85 Citation Subset: IM |
Affiliation:
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National Food Research Institute, Tsukuba, Ibaraki 305-8642, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Base Sequence Catalysis Chromatography, Gel Cloning, Molecular DNA Primers Dimerization Electrophoresis, Polyacrylamide Gel Genes, Bacterial Glutamate Decarboxylase / chemistry, genetics, isolation & purification, metabolism* Hydrogen-Ion Concentration Lactobacillus / enzymology*, genetics, metabolism Molecular Sequence Data Sequence Homology, Amino Acid Temperature gamma-Aminobutyric Acid / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 56-12-2/gamma-Aminobutyric Acid; EC 4.1.1.15/Glutamate Decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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