| Characterization of glucose transport by cultured rabbit kidney proximal convoluted and proximal straight tubule cells. | |
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MedLine Citation:
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PMID: 12197774 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rabbit kidney proximal convoluted tubule (RPCT) and proximal straight tubule (RPST) cells were independently isolated and cultured. The kinetics of the sodium-dependent glucose transport was characterized by determining the uptake of the glucose analog alpha-methylglucopyranoside. Cell culture and assay conditions used in these experiments were based on previous experiments conducted on the renal cell line derived from the whole kidney of the Yorkshire pig (LLC-PK1). Results indicated the presence of two distinct sodium-dependent glucose transporters in rabbit renal cells: a relatively high-capacity, low-affinity transporter (V(max) = 2.28 +/- 0.099 nmoles/mg protein min, Km = 4.1 +/- 0.27 mM) in RPCT cells and a low-capacity, high-affinity transporter (V(max) = 0.45 +/- 0.076 nmoles/mg protein min, K(m) = 1.7 +/- 0.43 mM) in RPST cells. A relatively high-capacity, low-affinity transporter (V(max) = 1.68 +/- 0.215 nmoles/mg protein min, Km = 4.9 +/- 0.23 mM) was characterized in LLC-PK1 cells. Phlorizin inhibited the uptake of alpha-methylglucopyranoside in proximal convoluted, proximal straight, and LLC-PK1 cells by 90, 50, and 90%, respectively. Sodium-dependent glucose transport in all three cell types was specific for hexoses. These data are consistent with the kinetic heterogeneity of sodium-dependent glucose transport in the S1-S2 and S3 segments of the mammalian renal proximal tubule. The RPCT-RPST cultured cell model is novel, and this is the first report of sodium-dependent glucose transport characterization in primary cultures of proximal straight tubule cells. Our results support the use of cultured monolayers of RPCT and RPST cells as a model system to evaluate segment-specific differences in these renal cell types. |
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Authors:
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Pedro L Del Valle; Anna Trifillis; Charles E Ruegg; Andrew S Kane |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: In vitro cellular & developmental biology. Animal Volume: 38 ISSN: 1071-2690 ISO Abbreviation: In Vitro Cell. Dev. Biol. Anim. Publication Date: 2002 Apr |
Date Detail:
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Created Date: 2002-08-28 Completed Date: 2003-02-12 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9418515 Medline TA: In Vitro Cell Dev Biol Anim Country: United States |
Other Details:
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Languages: eng Pagination: 218-27 Citation Subset: IM |
Affiliation:
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Department of Pathology, School of Medicine, University of Maryland, Baltimore 21201, USA. Pedro.Delvalle@na.amedd.army.mil |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport Cell Culture Techniques / methods Cell Line Cells, Cultured Glucose / metabolism* Kidney Tubules, Proximal / cytology*, physiology Kinetics Monosaccharide Transport Proteins / metabolism* Rabbits Xenopus laevis |
| Grant Support | |
ID/Acronym/Agency:
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R01ES06217/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Monosaccharide Transport Proteins; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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