Document Detail


Characterization of a glucocorticoid-sensitive human lymphoid cell line.
MedLine Citation:
PMID:  269011     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A human lymphoblastoid cell line (CEM), the growth of which is inhibited by glucocorticoids, is described. Although growth of the original uncloned cell line is only slightly retarded by dexamethasone, sensitive clones were isolated in which growth is completely blocked by 2 to 3 days of exposure to 10(-6) M dexamethasone. After 4 to 5 days, these cells become pyknotic and lyse. The inhibitory affect of dexamethasone first become apparent in suspension culture at a concentration of about 5 X 10(-8) M. Receptor analysis showed the presence of specific glucocorticoid receptors with an apparent dissociation constant for dexamethasone of about 1.3 X 10(-8) M both in the sensitive and in one resistant clone analyzed. Ability to displace dexamethasone from the receptor is correlated with the known glucocorticoid activity of all steroids tested, as is their ability to inhibit cloning of sensitive cells in agarose. These results indicate that the specificity of inhibitory effects is related to receptor specificity of inhibitory effects is related to receptor specificity. Dexamethasone is a potent inhibitor when cells are cloned in agarose, having a marked effect even at a concentration of 7 X 10(-9) M. CEM cells thus provide human cell lines suitable for in vitro analysis of steroid effects on leukemic cells.
Authors:
M R Norman; E B Thompson
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer research     Volume:  37     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1977 Oct 
Date Detail:
Created Date:  1977-11-30     Completed Date:  1977-11-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3785-91     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division / drug effects
Cell Line
Clone Cells / drug effects
Dexamethasone / metabolism,  pharmacology
Humans
Hydrocortisone / pharmacology
Leukemia, Experimental / metabolism,  pathology
Leukemia, Lymphoid / metabolism*,  pathology
Prednisolone / pharmacology
Receptors, Glucocorticoid*
Receptors, Steroid*
Chemical
Reg. No./Substance:
0/Receptors, Glucocorticoid; 0/Receptors, Steroid; 50-02-2/Dexamethasone; 50-23-7/Hydrocortisone; 50-24-8/Prednisolone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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